Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 9/CA9
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Carbonic Anhydrase 9/CA9 antibody
See all Carbonic Anhydrase 9/CA9 primary antibodies
DescriptionRabbit polyclonal to Carbonic Anhydrase 9/CA9
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Carbonic Anhydrase 9/CA9 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
- Carbonic Anhydrase 9/CA9 recombinant protein This antibody gave a positive result in IF in the following methanol fixed cell lines: DU145.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab10471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 49.7 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionReversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Tissue specificityExpressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain.
modificationsAsn-346 bears high-mannose type glycan structures.
Cellular localizationNucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
- Information by UniProt
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
ab10471 staining Carbonic Anhydrase 9/CA9 in Human kidney tissue sections by IHC-P (Formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5 minutes of peroxidase block followed by 10 minutes of protein block at 20°C; antigen retrieval was heat mediated in retrieval solution. Samples were incubated with primary antibody (1/250 in antibody diluent) for 45 minutes at 20°C. An undiluted HRP-conjugated polymer goat anti-mouse/rabbit IgG polyclonal was used as secondary antibody.
Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471) at 1 µg/ml + Carbonic Anhydrase IX recombinant protein at 0.1 µg/ml
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49.7 kDa
Observed band size: 53,54 kDa why is the actual band size different from the predicted?
This antibody was tested on the Carbonic Anhydrase IX recombinant protein which migrates as a doublet at 53 and 54kDa.
ICC/IF image of ab10471 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10471 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab10471 has not yet been referenced specifically in any publications.