Western blot abreview for Anti-Carbonic Anhydrase IX antibody

Western blot

Mouse Tissue lysate - whole (Cortical lysates)

20 µg

Cortical lysates

Reduced Denaturing (10% gel)

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

1/5000

18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 0.05% tween-20 and 5% milk

Non-Abcam antibody was used: Alexafluor680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680

1/5000

Odyssey

2 minute(s) and 30 second(s)

Odyssey detection system used (intensity 2.5). Nitrocellulose membrane used, lysis buffer was 50mM Tris-HCL, 10mM EDTA and 5mM EGTA (pH 7.6). Roche complete protease inhibitor tablets used.

Mouse and rat were not a recommended species for ab10471 and based on the low homology between human and mouse and rat Carbonic Anhydrase IX protein it might be possible that this antibody it is not suitable for use in mouse and rat. Abcam would like to recommend that researches interested in detecting Carbonic Anhydrase IX in mouse and rat would use a different antibody. Thank you for your valuable feedback, we hope this will be useful for other customers