Overview

  • Product name

    Anti-Carbonic Anhydrase 9/CA9 antibody
    See all Carbonic Anhydrase 9/CA9 primary antibodies
  • Description

    Rabbit polyclonal to Carbonic Anhydrase 9/CA9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Carbonic Anhydrase 9/CA9 aa 1-168. The exact sequence is proprietary.
    Database link: Q16790

  • Positive control

    • HepG2 cell lysate, treated with 200µM CoCl2 for 24hr; Human cervical carcinoma tissue; CoCl2- treated A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab184006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
  • Tissue specificity

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.
  • Post-translational
    modifications

    Asn-346 bears high-mannose type glycan structures.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA-IX antibody
    • CA9 antibody
    • CAH9_HUMAN antibody
    • CAIX antibody
    • Carbonate dehydratase IX antibody
    • Carbonic anhydrase 9 antibody
    • Carbonic anhydrase IX antibody
    • Carbonic dehydratase antibody
    • G250 antibody
    • Membrane antigen MN antibody
    • MN antibody
    • P54/58N antibody
    • pMW1 antibody
    • RCC associated protein G250 antibody
    • RCC-associated antigen G250 antibody
    • Renal cell carcinoma-associated antigen G250 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution.

  • All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate, untreated
    Lane 2 : HepG2 whole cell lysate, treated with 200 µM CoCl2 for 24 hr

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa



    10% SDS PAGE

  • Immunofluorescent analysis of A431 cells labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution. A431 cells 
    were fixed in ice-cold MeOH for 5 min. Cells were treated with 200 μM CoCl2  (right) or untreated (left)  
    Green: Carbonic Anhydrase 9/CA9 protein showing membrane staining.
    Blue: Hoechst 33342.

References

This product has been referenced in:

  • Maybin JA  et al. Hypoxia and hypoxia inducible factor-1a are required for normal endometrial repair during menstruation. Nat Commun 9:295 (2018). Read more (PubMed: 29362355) »
  • Qi Q  et al. Co-amplification of phosphoinositide 3-kinase enhancer A and cyclin-dependent kinase 4 triggers glioblastoma progression. Oncogene 36:4562-4572 (2017). Read more (PubMed: 28368413) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% gel, TGS buffer)
Sample
Mouse Tissue lysate - whole (Heart left ventricule)
Specification
Heart left ventricule
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 19 2014

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