Key features and details
- Mouse monoclonal [CA9/781] to Carbonic Anhydrase 9/CA9
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Carbonic Anhydrase 9/CA9 antibody [CA9/781]
See all Carbonic Anhydrase 9/CA9 primary antibodies
DescriptionMouse monoclonal [CA9/781] to Carbonic Anhydrase 9/CA9
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Horse
Recombinant full length protein corresponding to Human Carbonic Anhydrase 9/CA9 aa 1-459.
Database link: Q16790
- WB: HCT116 cell lysate. IHC-P: Human renal cell carcinoma.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab216021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 49 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.5-1µg for 106 cells.|
FunctionReversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Tissue specificityExpressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain.
modificationsAsn-346 bears high-mannose type glycan structures.
Cellular localizationNucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
- Information by UniProt
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
Anti-Carbonic Anhydrase 9/CA9 antibody [CA9/781] (ab216021) at 1 µg/ml + HCT116 cell lysate
Predicted band size: 49 kDa
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human renal cell carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab216021 at 1 μg/ml dilution, followed by conjugation to the secondary antibody and DAB staining.
ab216021 has been referenced in 1 publication.
- Zhou L et al. Biphasic squamoid alveolar renal carcinoma with positive CD57 expression: A clinicopathologic study of three cases. Pathol Int 69:519-525 (2019). PubMed: 31369187