Overview

  • Product name

    Anti-Carbonic Anhydrase 9/CA9 antibody [CA9/781]
    See all Carbonic Anhydrase 9/CA9 primary antibodies
  • Description

    Mouse monoclonal [CA9/781] to Carbonic Anhydrase 9/CA9
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse
  • Immunogen

    Recombinant full length protein corresponding to Human Carbonic Anhydrase 9/CA9 aa 1-459.
    Database link: Q16790

  • Positive control

    • WB: HCT116 cell lysate. IHC-P: Human renal cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab216021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 49 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5-1µg for 106 cells.

Target

  • Function

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
  • Tissue specificity

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.
  • Post-translational
    modifications

    Asn-346 bears high-mannose type glycan structures.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA-IX antibody
    • CA9 antibody
    • CAH9_HUMAN antibody
    • CAIX antibody
    • Carbonate dehydratase IX antibody
    • Carbonic anhydrase 9 antibody
    • Carbonic anhydrase IX antibody
    • Carbonic dehydratase antibody
    • G250 antibody
    • Membrane antigen MN antibody
    • MN antibody
    • P54/58N antibody
    • pMW1 antibody
    • RCC associated protein G250 antibody
    • RCC-associated antigen G250 antibody
    • Renal cell carcinoma-associated antigen G250 antibody
    see all

Images

  • Anti-Carbonic Anhydrase 9/CA9 antibody [CA9/781] (ab216021) at 1 µg/ml + HCT116 cell lysate

    Predicted band size: 49 kDa

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human renal cell carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab216021 at 1 μg/ml dilution, followed by conjugation to the secondary antibody and DAB staining.

References

This product has been referenced in:

  • Zhou L  et al. Biphasic squamoid alveolar renal carcinoma with positive CD57 expression: A clinicopathologic study of three cases. Pathol Int 69:519-525 (2019). Read more (PubMed: 31369187) »
See 1 Publication for this product

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