Recombinant
RabMAb

Recombinant Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (ab108351)

Overview

  • Product name

    Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)]
    See all Carbonic Anhydrase 9/CA9 primary antibodies
  • Description

    Rabbit monoclonal [EPR4151(2)] to Carbonic Anhydrase 9/CA9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Carbonic Anhydrase 9/CA9 aa 200-300 (extracellular). The exact sequence is proprietary.
    Database link: Q16790

  • Positive control

    • WB: HT-29 and human stomach lysates. IHC-P: Human stomach and renal cell carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
    • Tissue specificity

      Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
    • Sequence similarities

      Belongs to the alpha-carbonic anhydrase family.
      Contains 1 alpha-carbonic anhydrase domain.
    • Post-translational
      modifications

      Asn-346 bears high-mannose type glycan structures.
    • Cellular localization

      Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CA-IX antibody
      • CA9 antibody
      • CAH9_HUMAN antibody
      • CAIX antibody
      • Carbonate dehydratase IX antibody
      • Carbonic anhydrase 9 antibody
      • Carbonic anhydrase IX antibody
      • Carbonic dehydratase antibody
      • G250 antibody
      • Membrane antigen MN antibody
      • MN antibody
      • P54/58N antibody
      • pMW1 antibody
      • RCC associated protein G250 antibody
      • RCC-associated antigen G250 antibody
      • Renal cell carcinoma-associated antigen G250 antibody
      see all

    Images

    • All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (ab108351) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : Human stomach lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab108351 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human renal cell carcinoma tissue using ab108351 at 1/100

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Bi C  et al. High Beclin-1 and ARID1A expression corelates with poor survival and high recurrence in intrahepatic cholangiocarcinoma: a histopathological retrospective study. BMC Cancer 19:213 (2019). Read more (PubMed: 30849962) »
    • Kim SW  et al. Selecting patients for hyperthermia combined with preoperative chemoradiotherapy for locally advanced rectal cancer. Int J Clin Oncol 23:287-297 (2018). Read more (PubMed: 29134362) »
    See all 12 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Gastric cells)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization
    Yes - Triton 0.025%
    Specification
    Gastric cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 03 2015

    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Cancer Cell Lines)
    Specification
    Cancer Cell Lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Aug 05 2014

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Human Cell lysate - whole cell (Glioma)
    Specification
    Glioma
    Blocking step
    Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 27 2013

    Application
    Western blot
    Loading amount
    30000 cells
    Gel Running Conditions
    Reduced Denaturing (11)
    Sample
    Human Cell lysate - whole cell (MCF-7 cells (human breast adenocarcinoma cell line)
    Specification
    MCF-7 cells (human breast adenocarcinoma cell line
    Treatment
    0.5% oxygen for 16hrs
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Nov 05 2013

    Question
    Answer

    Thank you for contacting us.

    We have several HIF antibodies in our catalog that react with mouse. For example, ab8366: HIF1 alpha, ab199: HIF2 alpha, ab10134: HIF3 alpha, ab465: HIF1 beta. If you could let me know which HIF you are looking for and what application it would be used for, I would be happy to help you narrow down the results.

    While we do not have any antibodies to Carbonic Anhydrase IX that are known to react with mouse, the antibodies ab108351 and ab15086 are likely to show some cross-reactivity due to sequence homology. If you would like to test either of these antibodies for use in mouse, I would be happy to offer you our 100% testing discount program. If you purchase the antibody for full price and submit an Abreview with your mouse results, you will receive a discount code that can be redeemed for a free primary antibody on a future order.

    I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

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