Recombinant
RabMAb

Recombinant Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)

Rabbit recombinant monoclonal Carbonic Anhydrase IX antibody [SP106]. Validated in IHC, Flow Cyt and tested in Human.

Overview

  • Product name

    Anti-Carbonic Anhydrase 9/CA9 antibody [SP106]
    See all Carbonic Anhydrase 9/CA9 primary antibodies
  • Description

    Rabbit monoclonal [SP106] to Carbonic Anhydrase 9/CA9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Carbonic Anhydrase 9/CA9 aa 400 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: Q16790

  • Positive control

    • Human stomach tissue; Ramos cell lysate
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab105226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
  • Tissue specificity

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.
  • Post-translational
    modifications

    Asn-346 bears high-mannose type glycan structures.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA-IX antibody
    • CA9 antibody
    • CAH9_HUMAN antibody
    • CAIX antibody
    • Carbonate dehydratase IX antibody
    • Carbonic anhydrase 9 antibody
    • Carbonic anhydrase IX antibody
    • Carbonic dehydratase antibody
    • G250 antibody
    • Membrane antigen MN antibody
    • MN antibody
    • P54/58N antibody
    • pMW1 antibody
    • RCC associated protein G250 antibody
    • RCC-associated antigen G250 antibody
    • Renal cell carcinoma-associated antigen G250 antibody
    see all

Images

  • Flow cytometric analysis of rabbit anti-Carbonic Anhydrase 9/CA9 (SP106) antibody ab105226 (1/100) in HT29  cells (green) compared to negative control of rabbit IgG (blue).

  • ab105226, at 1/100 dilution, staining Carbonic Anhydrase 9/CA9 in formalin-fixed, paraffin-embedded Human stomach tissue by Immunohistochemistry.

References

ab105226 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Answer

Merci de nous avoir contactés. Les anticorps primaires Abcam sont livrés sans diluent ni anticorps secondaire. J'ai retrouvé la commande passée par ****. Cette commande concerne en effet 2 anticorps primaires, l'anti-HIF1 alpha antibody [ESEE122]référence ab8366 et l'anti-Carbonic Anhydrase IX antibody [SP106]référence ab105226. Le lien vers la liste des anticorps secondaires compatibles avec ab8366 (Mouse IgG1)est : https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG1&pt=7&sk=targsp&sv=2&sn=Mouse&l=0&fViewMore=1 Le lien vers la liste des anticorps secondaires compatibles avec ab105226 (Rabbit IgG) est : https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG&pt=7&sk=targsp&sv=2&sn=Rabbit&l=0&fViewMore=1 Vous pouvez affiner votre recherche en utilisant les filtres à gauche de la liste (application, conjugué, etc). La solution de dilution dépendra de l'application testée. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions. Bonne et heureuse année 2012 à vouségalement.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up