Overview

  • Product name
    Anti-Carbonic Anhydrase I antibody
    See all Carbonic Anhydrase I primary antibodies
  • Description
    Goat polyclonal to Carbonic Anhydrase I
  • Host species
    Goat
  • Specificity
    No cross-reactivity to carbonic anhydrase II.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Carbonic Anhydrase I [Human Erythrocytes]

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.212% Potassium phosphate
  • Purity
    Whole antiserum
  • Purification notes
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
ELISA 1/1000 - 1/5000.

Target

  • Function
    Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
  • Sequence similarities
    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 1 antibody
    • CA I antibody
    • CA-I antibody
    • CA1 antibody
    • CAB antibody
    • CAH1_HUMAN antibody
    • CAI antibody
    • CAN antibody
    • Car 1 antibody
    • Car1 antibody
    • Carbonate dehydratase I antibody
    • Carbonic anhydrase 1 antibody
    • Carbonic anhydrase A antibody
    • Carbonic anhydrase B antibody
    • Carbonic anhydrase B, formerly antibody
    • Carbonic anhydrase I antibody
    • Carbonic dehydratase antibody
    • ECK0125 antibody
    • JW0122 antibody
    • yadF antibody
    see all

Images

  • WB using ab6618. Review by Morena Cobbs submitted 7 January 2004.


    WB using ab6618. Review by Morena Cobbs submitted 7 January 2004

References

This product has been referenced in:
  • Guo F  et al. Inhibition of carbonic anhydrase reduces brain injury after intracerebral hemorrhage. Transl Stroke Res 3:130-137 (2012). WB ; Rat . Read more (PubMed: 22400066) »
  • Chang X  et al. Increased expression of carbonic anhydrase I in the synovium of patients with ankylosing spondylitis. BMC Musculoskelet Disord 11:279 (2010). WB ; Human . Read more (PubMed: 21143847) »

See all 3 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain tissue (not perfused / perfused))
Loading amount
30 µg
Specification
brain tissue (not perfused / perfused)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Username

Dr. Dominik Schelshorn

Verified customer

Submitted Jan 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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