Overview

  • Product name
    Anti-Carbonic Anhydrase II antibody
    See all Carbonic Anhydrase II primary antibodies
  • Description
    Rabbit polyclonal to Carbonic Anhydrase II
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Positive control
    • WB: carbonic Anhydrase II IHC-P: Mouse eye tissue; Human kidney tissue.
  • General notes

    This product is the 100 µg version of ab6621.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 16586439
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
  • Involvement in disease
    Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
  • Sequence similarities
    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 2 antibody
    • CA II antibody
    • CA-II antibody
    • Ca2 antibody
    • CAC antibody
    • CAH2_HUMAN antibody
    • CAII antibody
    • Car 2 antibody
    • Car2 antibody
    • Carbonate dehydratase II antibody
    • Carbonic anhydrase 2 antibody
    • Carbonic anhydrase B antibody
    • Carbonic anhydrase C antibody
    • Carbonic anhydrase C, formerly antibody
    • Carbonic anhydrase II antibody
    • Carbonic dehydratase antibody
    • epididymis luminal protein 76 antibody
    • Epididymis secretory protein Li 282 antibody
    • HEL-76 antibody
    • HEL-S-282 antibody
    see all

Images

  • Anti-Carbonic Anhydrase II antibody (ab191343) at 1/1000 dilution + Carbonic Anhydrase II at 0.05 µg

    Secondary
    Rabbit Peroxidase at 1/40000 dilution
  • Formalin-fixed, paraffin-embedded human kidney tissue stained for Carbonic Anhydrase II using ab191343 at 4 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded mouse eye tissue stained for Carbonic Anhydrase II using ab191343 at 4 μg/ml in immunohistochemical analysis.

References

This product has been referenced in:
  • Tachibana H  et al. Carbonic anhydrase 2 is a novel invasion-associated factor in urinary bladder cancers. Cancer Sci 108:331-337 (2017). Read more (PubMed: 28004470) »
  • Pandya M  et al. Integrative Temporo-Spatial, Mineralogic, Spectroscopic, and Proteomic Analysis of Postnatal Enamel Development in Teeth with Limited Growth. Front Physiol 8:793 (2017). Read more (PubMed: 29114228) »

See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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