Product nameAnti-Carbonic Anhydrase II antibody [EPR5195]
See all Carbonic Anhydrase II primary antibodies
DescriptionRabbit monoclonal [EPR5195] to Carbonic Anhydrase II
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to a sequence from the C terminus of Human Carbonic Anhydrase II.
- Human fetal brain, Caco 2, 293T, and Human heart lysates; Human kidney and brain tissues.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.42 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionEssential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
Involvement in diseaseDefects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
- Information by UniProt
- CA 2 antibody
- CA II antibody
- CA-II antibody
ab124687, at 1/250 dilution, staining Carbonic Anhydrase II in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Carbonic Anhydrase II knockout HAP1 cell lysate (40 µg)
Lane 3: A431 cell lysate (40 µg)
Lane 4: HEK293 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124687 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124687 was shown to specifically react with Carbonic Anhydrase II when Carbonic Anhydrase II knockout samples were used. Wild-type and Carbonic Anhydrase II knockout samples were subjected to SDS-PAGE. Ab124687 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Carbonic Anhydrase II antibody [EPR5195] (ab124687) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Caco 2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human heart tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
ab124687, at 1/250 dilution, staining Carbonic Anhydrase II in paraffin-embedded Human brain tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Li H et al. Expression and localization of Forkhead transcription factor A1 in the three-dimensional reconstructed eccrine sweat glands. Acta Histochem 120:520-524 (2018). Read more (PubMed: 29909922) »
- Tachibana H et al. Carbonic anhydrase 2 is a novel invasion-associated factor in urinary bladder cancers. Cancer Sci 108:331-337 (2017). Read more (PubMed: 28004470) »