Carboxy-DCFDA N-succinimidyl ester, Fluorogenic esterase substrate (ab145286)

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Key features and details

  • Cell-permeable, fluorogenic esterase substrate
  • CAS Number: 147265-60-9
  • Purity: > 96%
  • Soluble in DMSO
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    Carboxy-DCFDA N-succinimidyl ester, Fluorogenic esterase substrate

  • Description

    Cell-permeable, fluorogenic esterase substrate

  • Biological description

    Cell-permeable, fluorogenic esterase substrate. Becomes hydrolyzed intracellularly to green fluorophore DCFA (ab145439) and covalently bonds to proteins.

  • Purity

    > 96%

  • CAS Number

    147265-60-9

  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    495nm

  • Emission

    529nm

  • Chemical name

    5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester

  • Molecular weight

    626.35

  • Molecular formula

    C29H17Cl2NO11

  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.

  • Solubility overview

    Soluble in DMSO

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

  • Research areas

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab145286? Please let us know so that we can cite the reference in this datasheet.

ab145286 has been referenced in 2 publications.

  • Rzymski P  et al. The chemistry and toxicity of discharge waters from copper mine tailing impoundment in the valley of the Apuseni Mountains in Romania. Environ Sci Pollut Res Int 24:21445-21458 (2017). PubMed: 28744684
  • Daubney J  et al. Cardioprotective and cardiotoxic effects of quercetin and two of its in vivo metabolites on differentiated h9c2 cardiomyocytes. Basic Clin Pharmacol Toxicol 116:96-109 (2015). PubMed: 25203460

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