Key features and details
- Rabbit polyclonal to Carboxymethyl Lysine
- Suitable for: ELISA, WB, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Carboxymethyl Lysine antibody
See all Carboxymethyl Lysine primary antibodies
DescriptionRabbit polyclonal to Carboxymethyl Lysine
SpecificityThis antibody specifically binds to Carboxy methyl Lysine modified proteins.
Tested applicationsSuitable for: ELISA, WB, IHC-Frmore details
Species reactivityReacts with: Species independent
Carboxy methyl Lysine conjugated to KLH.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.015% EDTA, 0.44% Sodium chloride, 30% Glycerol, 1.23% Sodium phosphate
Concentration information loading...
Purification notesThis antibody was purified by Carboxy methyl Lysine protein Sepharose affinity column.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab27684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/2500 - 1/20000.|
|WB||1/2500 - 1/20000.|
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceN epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
- CML antibody
- N Epsilon (Carboxymethyl) Lysine antibody
All lanes : Anti-Carboxymethyl Lysine antibody (ab27684) at 1/1000 dilution (in PBST for 18 hours at 4°C)
Lane 1 : Whole tissue lysate of raw cashew nuts
Lane 2 : Whole tissue lysate of roasted cashew nuts
Lysates/proteins at 50 µg per lane.
All lanes : An HRP-conjugated Goat anti-rabbit polyclonal
Developed using the ECL technique.
Observed band size: 10-12 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 2% Milk for 30 minutes at 25°C
ab27684 staining Carboxymethyl Lysine in Mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A biotin-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab27684 has been referenced in 22 publications.
- Prosser CG et al. Ne-carboxymethyllysine in nutritional milk formulas for infants. Food Chem 274:886-890 (2019). PubMed: 30373023
- Teissier T et al. Knockout of receptor for advanced glycation end-products attenuates age-related renal lesions. Aging Cell 18:e12850 (2019). PubMed: 30794349
- Liu YH et al. Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in D-galactose-induced BALB/c mice in vivo. Bot Stud 60:9 (2019). PubMed: 31250143
- Gasparotto J et al. Receptor for advanced glycation end products mediates sepsis-triggered amyloid-ß accumulation, Tau phosphorylation, and cognitive impairment. J Biol Chem 293:226-244 (2018). PubMed: 29127203
- Balansin Rigon R et al. Ultrastructural and Molecular Analysis of Ribose-Induced Glycated Reconstructed Human Skin. Int J Mol Sci 19:N/A (2018). PubMed: 30413126