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Thank you for your reply. I have another question regarding this CML
antibody ab30922. Could you tell me please if it is possible to use it in
Immunohistochemistry in paraffin without antigen retrieval?
I saw on the product leaflet of another polyclonal anti-CML antibody you
adviced your customer to use it with hot antigen retrieval with paraffin.
I am looking foreward your answer.
Asked on Jan 24 2012
Thank you for contacting us.
We normally use heat mediated antigen retrieval in IHC-P. The extra information when we have, is always written as application note under the application. I have checked the data currently we have for this antibody and unfortunately we haven't tested this antibody in IHC-P in our own lab. The IHC-P application is added because of an Abreview which is also published online. I also have attached a publication which mentions successful use of this ab in IHC-P. In both case the heat mediated antigen retrieval method has been avoided.
I presume the heat might have damaging effect on the structure CML thereby giving false results. I therefore can suggest using enzymatic retrieval method or following the protocol as mentioned in the attached publication.
If you are interested I can provide you abreview discount code for using this antibody in IHC-P. Using the discount code you can avail a free antibody in return of an Abreview. Please click on the link for more details;
Let me know if you are interested in my offer.
Answered on Jan 24 2012