Overview

  • Product name
    Anti-Carboxymethyl Lysine antibody [NF-1G]
    See all Carboxymethyl Lysine primary antibodies
  • Description
    Mouse monoclonal [NF-1G] to Carboxymethyl Lysine
  • Host species
    Mouse
  • Specificity
    This antibody is suitable for the detection of N Epsilon (Carboxymethyl) Lysine (CML) in tissues and tissue extracts.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    N Epsilon (Carboxymethyl) Lysine (CML)-HSA.

Properties

Applications

Our Abpromise guarantee covers the use of ab30917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 0.5 µg/ml.
IHC-Fr Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • Alternative names
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

Images

  • ab30917 staining Carboxymethyl Lysine in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and embedded in paraffin. Samples were incubated with primary antibody (1/125 in PBS + 1% BSA) for overnight at 4°C. A Biotin-conjugated secondary was used with DAB visualisation the samples were then counterstained with haematoxylin.

References

This product has been referenced in:
  • Zhuang A  et al. Increased liver AGEs induce hepatic injury mediated through an OST48 pathway. Sci Rep 7:12292 (2017). IHC . Read more (PubMed: 28947796) »
  • Crisan M  et al. Expression of Advanced Glycation End-Products on Sun-Exposed and Non-Exposed Cutaneous Sites during the Ageing Process in Humans. PLoS One 8:e75003 (2013). IHC-P ; Human . Read more (PubMed: 24116020) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - whole (cortex and hippocampus)
Specification
cortex and hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RM°C

Abcam user community

Verified customer

Submitted Mar 28 2014

Answer

I'm sorry I was not clearer in my explanation. Both ab30917 and ab30922 should be suitable for you experiment. The reason why we sometimes have what seems to be duplicates within our catalogue is that in order to make sure we have a continuous supply of an antibody which we outsouce, we also supply a back up, in case one of the suppliers is unable to continue providing us with the antibody. So if we are unable to continue selling ab30917 we would be able to provide ab30922 for our customers and vice versa. I hope this explanation helps. I wish you well in your experiments. If you have any further questions please do not hesitate to ask.

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Answer

Thank you for contacting us. The two antibodies (ab30917 and ab30922) are both mouse monoclonal antibodies directed against N Epsilon (Carboxymethyl) Lysine (CML). They have both been tested with ELISA, IHP (frozen sections and paraffin). They are of the same concentration (0.250 mg/ml) and have been purified in the same way (Protein G purified). The only difference is that the clones are different (ab30917 clone number CMS-10 and ab30922 and NF-1G). This means the antibodies come from different lines of hybridoma. This should have no effect on the performance on the antibody relative to the other one. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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