Overview

  • Product name
    Anti-Carboxypeptidase A antibody [EPR12086]
    See all Carboxypeptidase A primary antibodies
  • Description
    Rabbit monoclonal [EPR12086] to Carboxypeptidase A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Carboxypeptidase A aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P15085

  • Positive control
    • Human, mouse and rat pancreas lysates; Human pancreas tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.

WB 1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    Images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Carboxypeptidase A1 using ab173283 at a 1/250 dilution.

    • ab173283 showing +ve staining in Human normal pancreas tissue.

    • ab173283 showing -ve staining in Human breast carcinoma tissue.
    • ab173283 showing -ve staining in Human normal uterus tissue.
    • ab173283 showing -ve staining in Human normal spleen tissue.
    • ab173283 showing -ve staining in Human cervical carcinoma tissue.
    • ab173283 showing -ve staining in Human normal brain tissue.
    • All lanes : Anti-Carboxypeptidase A antibody [EPR12086] (ab173283) at 1/1000 dilution

      Lane 1 : Human pancreas lysate
      Lane 2 : mouse pancreas lysate
      Lane 3 : rat pancreas lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa

    References

    This product has been referenced in:
    • Smith MD  et al. CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis. Dev Cell 44:217-232.e11 (2018). Read more (PubMed: 29290589) »
    • Song Y  et al. Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma. Transl Oncol 11:691-699 (2018). WB ; Human . Read more (PubMed: 29631213) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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