Recombinant
RabMAb

Recombinant Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)

Overview

  • Product name

    Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)]
    See all Carboxypeptidase A1+A2+B primary antibodies
  • Description

    Rabbit monoclonal [EPR12087(B)] to Carboxypeptidase A1+A2+B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Carboxypeptidase A1+A2+B aa 350-450. The exact sequence is proprietary. UniProt accessions: P15085, P15086, P48052.

  • Positive control

    • Human, mouse and rat pancreas lysates; Human pancreas tissue; BxPC3 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/250 - 1/500.
ICC/IF 1/50 - 1/100.

Images

  • Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/5000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunofluorescent analysis of BxPC3 cells (paraformaldehyde-fixed, 4%) labeling Carboxypeptidase A with ab181146 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • All lanes : Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/1000 dilution

    Lane 1 : Rat pancreas lysate
    Lane 2 : Mouse pancreas lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab181146 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

References

ab181146 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181146.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up