Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12087(B)] to Carboxypeptidase A1+A2+B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)]
See all Carboxypeptidase A1+A2+B primary antibodies
DescriptionRabbit monoclonal [EPR12087(B)] to Carboxypeptidase A1+A2+B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Carboxypeptidase A1+A2+B aa 350-450. The exact sequence is proprietary. UniProt accessions: P15085, P15086, P48052.
- Human, mouse and rat pancreas lysates; Human pancreas tissue; BxPC3 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/5000 dilution + Human pancreas lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDa
Immunofluorescent analysis of BxPC3 cells (paraformaldehyde-fixed, 4%) labeling Carboxypeptidase A with ab181146 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
All lanes : Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/1000 dilution
Lane 1 : Rat pancreas lysate
Lane 2 : Mouse pancreas lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDa
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab181146 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab181146 has not yet been referenced specifically in any publications.