Anti-Carboxypeptidase B/CPB antibody (ab153716)
Key features and details
- Rabbit polyclonal to Carboxypeptidase B/CPB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Carboxypeptidase B/CPB antibody
See all Carboxypeptidase B/CPB primary antibodies -
Description
Rabbit polyclonal to Carboxypeptidase B/CPB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Pig -
Immunogen
Recombinant fragment corresponding to Human Carboxypeptidase B/CPB aa 144-417.
Database link: P15086 -
Positive control
- WB: A549, HeLa, AsPC-1, HepG2, transfected 293T and HCT116 whole cell lysates, mouse and rat tissue lysate; IHC-P: Human Ca922 xenograft, mouse and rat pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Pancreas. -
Sequence similarities
Belongs to the peptidase M14 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 403512 Dog
- Entrez Gene: 1360 Human
- Entrez Gene: 76703 Mouse
- Entrez Gene: 397341 Pig
- Entrez Gene: 24271 Rat
- Omim: 114852 Human
- SwissProt: P55261 Dog
- SwissProt: P15086 Human
see all -
Alternative names
- Carboxypeptidase B antibody
- Carboxypeptidase B1 (tissue) antibody
- Carboxypeptidase B1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded rat pancreas tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/1000 dilution. Antigen Retrieval performed using citrate buffer, pH 6.0, 15 min
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All lanes : Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : AsPC-1 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
All lanes : Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/5000 dilution
Lane 1 : Non-transfected 293T cell lysate
Lane 2 : Carboxypeptidase B/CPB transfected 293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/50000 dilution + Mouse pancreas tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/1000 dilution. Antigen Retrieval performed using citrate buffer, pH 6.0, 15 min
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Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/50000 dilution + Rat pancreas tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab153716 has not yet been referenced specifically in any publications.