Recombinant Anti-Carboxypeptidase B2 antibody [EPR7858] - BSA and Azide free (ab248612)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7858] to Carboxypeptidase B2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Carboxypeptidase B2 antibody [EPR7858] - BSA and Azide free
See all Carboxypeptidase B2 primary antibodies -
Description
Rabbit monoclonal [EPR7858] to Carboxypeptidase B2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab248612 is the carrier-free version of ab133679.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 3.67 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7858 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. |
Target
-
Function
Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. -
Tissue specificity
Plasma; synthesized in the liver. -
Sequence similarities
Belongs to the peptidase M14 family. -
Post-translational
modificationsN-glycosylated. N-glycan at Asn-108: Hex5HexNAc4. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 1361 Human
- Entrez Gene: 56373 Mouse
- Entrez Gene: 113936 Rat
- Omim: 603101 Human
- SwissProt: Q96IY4 Human
- SwissProt: Q9JHH6 Mouse
- SwissProt: Q9EQV9 Rat
- Unigene: 512937 Human
see all -
Alternative names
- 1110032P04Rik antibody
- 4930405E17Rik antibody
- bA139H14.2 antibody
see all
Images
-
All lanes : Anti-Carboxypeptidase B2 antibody [EPR7858] (ab133679) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 48 kDaThis data was developed using ab133679, the same antibody clone in a different buffer formulation.
-
This data was developed using ab133679, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab248612 has not yet been referenced specifically in any publications.