Overview

  • Product name
    Anti-Carboxypeptidase Y antibody
    See all Carboxypeptidase Y primary antibodies
  • Description
    Rabbit polyclonal to Carboxypeptidase Y
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Dot blot, IP, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Carboxypeptidase Y [Baker's Yeast]

Properties

Applications

Our Abpromise guarantee covers the use of ab34636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 60 kDa).
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.
  • Sequence similarities
    Belongs to the peptidase S10 family.
  • Post-translational
    modifications
    Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa).
  • Cellular localization
    Vacuole. Lysosome-like vacuoles.
  • Information by UniProt
  • Database links
    • Alternative names
      • carboxypeptidase C PRC1 antibody
      • Carboxypeptidase Y antibody
      • Carboxypeptidase YSCY antibody
      • CBPY_YEAST antibody
      • CPY antibody
      • LBC1 antibody
      • PRC1 antibody
      • Vacuolar carboxypeptidase Y antibody
      see all

    Images

    • Anti-Carboxypeptidase Y antibody (ab34636) at 1/5000 dilution + Cellular extracts at 10 µg

      Secondary
      HRP goat anti-rabbit IgG at 1/4000 dilution

      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 61 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Wang S  et al. Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum. Nat Commun 9:2939 (2018). Read more (PubMed: 30054465) »
    • Pfeiffer A  et al. A Complex of Htm1 and the Oxidoreductase Pdi1 Accelerates Degradation of Misfolded Glycoproteins. J Biol Chem 291:12195-207 (2016). Read more (PubMed: 27053108) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Answer

    Thank you for your reply.

    I would be happy to create a credit note/refund for your purchase of ab34636. In order to do this could you provide the PO used for that transaction? It is also very helpful for our internal testing if you could provide the details of your western blot protocol? Images are very helpful as well and I thank you for your offer of them.

    I hope this information is helpful to you and look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More
    Application
    Western blot
    Sample
    Pichia pastoris Cell lysate - whole cell (Yeast)
    Loading amount
    10 µg
    Specification
    Yeast
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Gaurav Agrawal

    Verified customer

    Submitted Mar 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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