Product nameAnti-Carboxypeptidase Y antibody
See all Carboxypeptidase Y primary antibodies
DescriptionRabbit polyclonal to Carboxypeptidase Y
Tested applicationsSuitable for: WB, Dot blot, IP, ELISAmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Carboxypeptidase Y [Baker's Yeast]
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Our Abpromise guarantee covers the use of ab34636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 60 kDa).|
|Dot blot||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
FunctionInvolved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.
Sequence similaritiesBelongs to the peptidase S10 family.
modificationsEnters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa).
Cellular localizationVacuole. Lysosome-like vacuoles.
- Information by UniProt
- carboxypeptidase C PRC1 antibody
- Carboxypeptidase Y antibody
- Carboxypeptidase YSCY antibody
Anti-Carboxypeptidase Y antibody (ab34636) at 1/5000 dilution + Cellular extracts at 10 µg
HRP goat anti-rabbit IgG at 1/4000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Wang S et al. Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum. Nat Commun 9:2939 (2018). Read more (PubMed: 30054465) »
- Pfeiffer A et al. A Complex of Htm1 and the Oxidoreductase Pdi1 Accelerates Degradation of Misfolded Glycoproteins. J Biol Chem 291:12195-207 (2016). Read more (PubMed: 27053108) »