Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/26] (ab201328)
Key features and details
- Mouse monoclonal [COL-1 + CEA31 + C66/26] to Carcino Embryonic Antigen CEA
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/26]
See all CEACAM5 primary antibodies -
Description
Mouse monoclonal [COL-1 + CEA31 + C66/26] to Carcino Embryonic Antigen CEA -
Host species
Mouse -
Specificity
ab201328 recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. ab201328 reacts with non-specific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
This product was produced with the following immunogens:
Tissue, cells or virus corresponding to Human Carcino Embryonic Antigen CEA. Human colon carcinoma extract (COL-1 & CEA31).
Recombinant full length protein corresponding to Human Carcino Embryonic Antigen CEA. (C66/261).
Database link: P06731 -
Positive control
- IHC-P: Human colon carcinoma tissue.
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General notes
Isotype - IgG2a (COL-1) + IgG1 (CEA31 & C66/261).
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Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab201328 is purified from Bioreactor Concentrate. -
Clonality
Monoclonal -
Clone number
COL-1 + CEA31 + C66/26 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For 30 min at RT. |
Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For 30 min at RT. |
Target
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Function
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. -
Tissue specificity
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. -
Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsComplex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1048 Human
- Omim: 114890 Human
- SwissProt: P06731 Human
- Unigene: 709196 Human
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Alternative names
- Carcinoembryonic antigen antibody
- Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
- CD66e antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab201328 has not yet been referenced specifically in any publications.