Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/26] (ab201328)

Overview

  • Product name

    Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/26]
    See all Carcino Embryonic Antigen CEA primary antibodies
  • Description

    Mouse monoclonal [COL-1 + CEA31 + C66/26] to Carcino Embryonic Antigen CEA
  • Host species

    Mouse
  • Specificity

    ab201328 recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. ab201328 reacts with non-specific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues.
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This product was produced with the following immunogens:
    Tissue, cells or virus corresponding to Human Carcino Embryonic Antigen CEA. Human colon carcinoma extract (COL-1 & CEA31).

    Recombinant full length protein corresponding to Human Carcino Embryonic Antigen CEA. (C66/261).
    Database link: P06731

  • Positive control

    • Human colon carcinoma tissue; MCF7 or 293T cells.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    Isotype - IgG2a (COL-1) + IgG1 (CEA31 & C66/261).

Properties

Applications

Our Abpromise guarantee covers the use of ab201328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IP Use at 2-4 µg/mg of lysate.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80-200 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For 30 min at RT.

IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

For 30 min at RT.

Target

  • Function

    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • Tissue specificity

    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Carcinoembryonic antigen antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
    • CD66e antibody
    • CEA antibody
    • Ceacam5 antibody
    • CEAM5_HUMAN antibody
    • DKFZp781M2392 antibody
    • Meconium antigen 100 antibody
    • OTTHUMP00000199032 antibody
    • OTTHUMP00000199033 antibody
    • OTTHUMP00000199034 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Carcino Embryonic Antigen CEA with ab201328 at 2 µg/ml.

References

ab201328 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab201328.
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