Key features and details
- Mouse monoclonal [COL-1 + CEA31 + C66/26] to Carcino Embryonic Antigen CEA
- Suitable for: Flow Cyt, ICC/IF, IP, WB, IHC-P, IHC-Fr
- Reacts with: Human
Product nameAnti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/26]
See all Carcino Embryonic Antigen CEA primary antibodies
DescriptionMouse monoclonal [COL-1 + CEA31 + C66/26] to Carcino Embryonic Antigen CEA
Specificityab201328 recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. ab201328 reacts with non-specific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues.
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
This product was produced with the following immunogens:
Tissue, cells or virus corresponding to Human Carcino Embryonic Antigen CEA. Human colon carcinoma extract (COL-1 & CEA31).
Recombinant full length protein corresponding to Human Carcino Embryonic Antigen CEA. (C66/261).
Database link: P06731
- Human colon carcinoma tissue; MCF7 or 293T cells.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Isotype - IgG2a (COL-1) + IgG1 (CEA31 & C66/261).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesab201328 is purified from Bioreactor Concentrate.
Clone numberCOL-1 + CEA31 + C66/26
Our Abpromise guarantee covers the use of ab201328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5-1µg for 106 cells.|
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IP||Use at 2-4 µg/mg of lysate.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80-200 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For 30 min at RT.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.
For 30 min at RT.
FunctionCell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Tissue specificityFound in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains.
modificationsComplex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
Cellular localizationCell membrane.
- Information by UniProt
- Carcinoembryonic antigen antibody
- Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
- CD66e antibody
ab201328 has not yet been referenced specifically in any publications.