Recombinant Anti-NLRC4 antibody [EPR19733] - BSA and Azide free (ab223143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19733] to NLRC4 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-NLRC4 antibody [EPR19733] - BSA and Azide free
See all NLRC4 primary antibodies -
Description
Rabbit monoclonal [EPR19733] to NLRC4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
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General notes
ab223143 is the carrier-free version of ab201792.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19733 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa). |
Target
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Function
Plays a role in the promotion of apoptosis. -
Tissue specificity
Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. -
Sequence similarities
Contains 1 CARD domain.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. Cytoplasmic filaments. - Information by UniProt
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Database links
- Entrez Gene: 58484 Human
- Entrez Gene: 268973 Mouse
- Omim: 606831 Human
- SwissProt: Q9NPP4 Human
- SwissProt: Q3UP24 Mouse
- Unigene: 574741 Human
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Alternative names
- and NACHT-containing protein antibody
- CARD 12 antibody
- CARD antibody
see all
Images
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All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201792).
Blocking/Dilution buffer: 5% NFDM/TBST.
NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab223143 has not yet been referenced specifically in any publications.