Overview

  • Product name

  • Description

    Rabbit polyclonal to CARD14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, Dot blot, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide corresponding to the internal portion of Human CARD14.

  • Positive control

    • Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 2.5 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 113 kDa).
Dot blot Use at an assay dependent concentration.
ELISA Use a concentration of 0.1 - 1 µg/ml.

Target

  • Function

    Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
  • Tissue specificity

    Expressed in placenta. Also detected in HeLaS3 cell line, but not in the other cancer cell lines tested. Expressed in epidermal keratinocytes.
  • Involvement in disease

    Defects in CARD14 are the cause of susceptibility to psoriasis type 2 (PSORS2) [MIM:602723]. A common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
    Defects in CARD14 are the cause of pityriasis rubra pilaris (PRP) [MIM:173200]. A rare, papulosquamous skin disease characterized by the appearance of keratotic follicular papules, well-demarcated salmon-colored erythematous plaques covered with fine powdery scales interspersed with distinct islands of uninvolved skin, and palmoplantar keratoderma. Most cases are sporadic. The rare familial cases show autosomal dominant inheritance with incomplete penetrance and variable expression. Familial PRP usually presents at birth or appears during the first years of life and runs a chronic course. It is characterized by prominent follicular hyperkeratosis, diffuse palmoplantar keratoderma, and erythema.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bcl10 interacting MAGUK protein 2 antibody
    • Bimp 2 antibody
    • Bimp2 antibody
    • CAR14_HUMAN antibody
    • CARD 14 antibody
    • CARD containing MAGUK 2 protein antibody
    • CARD containing MAGUK protein 2 antibody
    • Card maguk protein 2 antibody
    • CARD-containing MAGUK protein 2 antibody
    • CARD14 antibody
    • Carma 2 antibody
    • Carma2 antibody
    • Caspase recruitment domain containing protein 14 antibody
    • Caspase recruitment domain family member 14 antibody
    • Caspase recruitment domain protein 14 antibody
    • Caspase recruitment domain-containing protein 14 antibody
    • PRP antibody
    • PSORS2 antibody
    • PSS1 antibody
    see all

Images

  • Anti-CARD14 antibody (ab64366) at 1/200 dilution + Jurkat whole cell lysate at 15 µg

    Predicted band size: 113 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?

  • ab64366 at 10µg/ml staining CARD14 in Human placenta by Immunohistichemistry (frozen sections).Localization of the antibody can be seen as the precipitated red signal. A hematoxylin purple nuclear counterstain in placental trophoblasts has been used.

References

ab64366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. I was not able to obtain the exact sequence of the immunogen but I did receive a range that contains it: amino acids 410-435 of human CARD14, which is PPGVLKQEARTREPCPREKQRLVRMH I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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