Key features and details
- Rabbit polyclonal to CARD14
- Suitable for: IHC-Fr, WB, Dot blot
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CARD14 antibody
See all CARD14 primary antibodies
DescriptionRabbit polyclonal to CARD14
Tested applicationsSuitable for: IHC-Fr, WB, Dot blotmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Monkey
Synthetic peptide corresponding to the internal portion of Human CARD14.
- Jurkat whole cell lysate (ab7899).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 2.5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 113 kDa).|
|Dot blot||Use at an assay dependent concentration.|
FunctionActivates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Tissue specificityExpressed in placenta. Also detected in HeLaS3 cell line, but not in the other cancer cell lines tested. Expressed in epidermal keratinocytes.
Involvement in diseaseDefects in CARD14 are the cause of susceptibility to psoriasis type 2 (PSORS2) [MIM:602723]. A common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
Defects in CARD14 are the cause of pityriasis rubra pilaris (PRP) [MIM:173200]. A rare, papulosquamous skin disease characterized by the appearance of keratotic follicular papules, well-demarcated salmon-colored erythematous plaques covered with fine powdery scales interspersed with distinct islands of uninvolved skin, and palmoplantar keratoderma. Most cases are sporadic. The rare familial cases show autosomal dominant inheritance with incomplete penetrance and variable expression. Familial PRP usually presents at birth or appears during the first years of life and runs a chronic course. It is characterized by prominent follicular hyperkeratosis, diffuse palmoplantar keratoderma, and erythema.
Sequence similaritiesContains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
- Bcl10 interacting MAGUK protein 2 antibody
- Bimp 2 antibody
- Bimp2 antibody
Anti-CARD14 antibody (ab64366) at 1/200 dilution + Jurkat whole cell lysate at 15 µg
Predicted band size: 113 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ab64366 at 10µg/ml staining CARD14 in Human placenta by Immunohistichemistry (frozen sections).Localization of the antibody can be seen as the precipitated red signal. A hematoxylin purple nuclear counterstain in placental trophoblasts has been used.
ab64366 has not yet been referenced specifically in any publications.