Overview

  • Product name

    Anti-CARD15/NOD2 antibody [2D9]
    See all CARD15/NOD2 primary antibodies
  • Description

    Mouse monoclonal [2D9] to CARD15/NOD2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CARD15/NOD2 aa 1-350.

  • Positive control

    • This antibody gave a positive signal when tested against HEK293 overexpressing CARD15/NOD2 protein.It also gave a positive signal in FFPE human tonsil tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as CARD15

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    2D9
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
  • Tissue specificity

    Monocytes-specific.
  • Involvement in disease

    Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
    Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.
  • Sequence similarities

    Contains 2 CARD domains.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACUG antibody
    • Arthrocutaneouveal granulomatosis antibody
    • BLAU antibody
    • CARD15 antibody
    • Caspase recruitment domain family, member 15 antibody
    • Caspase recruitment domain protein 15 antibody
    • Caspase recruitment domain-containing protein 15 antibody
    • CD antibody
    • CLR16.3 antibody
    • IBD1 antibody
    • Inflammatory bowel disease protein 1 antibody
    • LRR containing protein antibody
    • NLR family, CARD domain containing 2 antibody
    • NLRC2 antibody
    • NOD like receptor C2 antibody
    • NOD2 antibody
    • NOD2 protein antibody
    • NOD2_HUMAN antibody
    • NOD2B antibody
    • nucleotide binding oligomerization domain 2 antibody
    • Nucleotide binding oligomerization domain containing 2 antibody
    • Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2 antibody
    • Nucleotide-binding oligomerization domain-containing protein 2 antibody
    • PSORAS1 antibody
    see all

Images

  • Anti-CARD15/NOD2 antibody [2D9] (ab31488) at 2 µg/ml + HEK 293 overexpressing CARD15/NOD2 at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 115 kDa
    Observed band size: 115 kDa


    Exposure time: 150 seconds
  • ab31488 staining CARD15/NOD2 in human tonsil epithelium tissue section by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer pH 6.0 for 20 minutes. Samples were incubated with primary antibody (1/150) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC image of CARD15/NOD2 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31488, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Xu X  et al. Knockdown of long noncoding RNA XIST alleviates oxidative low-density lipoprotein-mediated endothelial cells injury through modulation of miR-320/NOD2 axis. Biochem Biophys Res Commun 503:586-592 (2018). Read more (PubMed: 29902461) »
  • Yamada Y  et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). Read more (PubMed: 24626634) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsillar epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0, 20 min, 100C
Permeabilization
No
Specification
tonsillar epithelium
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 16 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 22 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (primary human macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
1e+006 cells
Specification
primary human macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up