Anti-CARD15/NOD2 antibody (ab115542)
Key features and details
- Rabbit polyclonal to CARD15/NOD2
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CARD15/NOD2 antibody
See all CARD15/NOD2 primary antibodies -
Description
Rabbit polyclonal to CARD15/NOD2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CARD15/NOD2 (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- Human small intestine tissue; HeLa cell lysate; HeLa cells.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 115 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 115 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). -
Tissue specificity
Monocytes-specific. -
Involvement in disease
Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement. -
Sequence similarities
Contains 2 CARD domains.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 64127 Human
- Omim: 605956 Human
- SwissProt: Q9HC29 Human
- Unigene: 592072 Human
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Alternative names
- ACUG antibody
- Arthrocutaneouveal granulomatosis antibody
- BLAU antibody
see all
Images
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Lane 1 : Anti-CARD15/NOD2 antibody (ab115542) at 2 µg/ml
Lane 2 : Anti-CARD15/NOD2 antibody (ab115542) at 4 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 115 kDa -
ab115542, at 3µg/ml, staining CARD15/NOD2 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115542 at 10µg/ml staining CARD15/NOD2 in HeLa cells by Immunocytochemistry.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab115542 has not yet been referenced specifically in any publications.