Key features and details
- Rabbit polyclonal to CARD15/NOD2
- Suitable for: WB, IHC-P, ICC/IF, ICC
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CARD15/NOD2 antibody
See all CARD15/NOD2 primary antibodies
DescriptionRabbit polyclonal to CARD15/NOD2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ICCmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CARD15/NOD2. The exact sequence is proprietary.
Database link: Q9HC29
- Human small intestine tissue. Jurkat cells. Jurkat cell lysate.
This product was previously labelled as CARD15
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 115 kDa.|
|IHC-P||Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|ICC||Use a concentration of 5 µg/ml.|
FunctionInduces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
Involvement in diseaseDefects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.
Sequence similaritiesContains 2 CARD domains.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
- Information by UniProt
- ACUG antibody
- Arthrocutaneouveal granulomatosis antibody
- BLAU antibody
Lane 1 : Anti-CARD15/NOD2 antibody (ab188646) at 1 µg/ml
Lane 2 : Anti-CARD15/NOD2 antibody (ab188646) at 2 µg/ml
Lane 3 : Anti-CARD15/NOD2 antibody (ab188646) at 4 µg/ml
All lanes : Jurkat cell lysate
Predicted band size: 115 kDa
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human small intestine sections labeling CARD15/NOD2 using ab188646 at 3 µg/ml.
Immunocytochemical/ Immunofluorescence analysis of Jurkat cells labeling CARD15/NOD2 using ab188646 at 20 µg/ml.
Immunocytochemical analysis of Jurkat cells labeling CARD15/NOD2 using ab188646 at 5 µg/ml.
ab188646 has not yet been referenced specifically in any publications.