Key features and details
- Rabbit polyclonal to CARD6
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CARD6 antibody
See all CARD6 primary antibodies
DescriptionRabbit polyclonal to CARD6
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CARD6 (internal sequence). The exact sequence is proprietary.
Database link: Q9BX69
- ICC/IF: HeLa cells. IHC-P: Human gastric cancer tissue. WB: A549 whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 116 kDa.|
FunctionMay be involved in apoptosis.
Sequence similaritiesContains 1 CARD domain.
- Information by UniProt
- CARD 6 antibody
- CARD containing inhibitor of Nod1 and Cardiak-induced NF kB activation antibody
- CARD6 antibody
Paraffin-embedded human gastric cancer tissue stained for CARD6 with ab227189 (1/500 dilution) in immunohistochemical analysis.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CARD6 (green) using ab227189 (1/500 dilution) in ICC/IF.
Counterstain: Hoechst 33342 (blue).
7.5% SDS PAGE
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227189 has not yet been referenced specifically in any publications.