Key features and details
- Rabbit polyclonal to CARD8
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CARD8 antibody
See all CARD8 primary antibodies
DescriptionRabbit polyclonal to CARD8
Specificityab24186 reacts with CARD8 (CARDINAL/TUCAN) protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to amino acids within the last 50 amino acids of the C terminus of human CARD8, GenBank accession AAG50014
- K562 cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab24186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).|
FunctionInhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.
Tissue specificityHigh expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.
Sequence similaritiesContains 1 CARD domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Apoptotic protein NDPP 1 antibody
- Apoptotic protein NDPP1 antibody
- CARD 8 antibody
Lane 1 : Anti-CARD8 antibody (ab24186) at 2 µg/ml (CARD8 expression in K562 cell lysate)
Lane 2 : Anti-CARD8 antibody (ab24186) at 4 µg/ml (CARD8 expression in K562 cell lysate)
All lanes :
Predicted band size: 49 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
A peptide blocking study was also done (data not shown) and the only bands that disappeared were around 45kDa. The other bands are thought to be non-specific, but more testing to confirm or refute this has not been performed.
ab24186 at 10µg/ml staining CARD8 in K562 cells by ICC/IF
ab24186 staining CARD8 in human monocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab24186 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.
ab24186 staining CARD8 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in 2% BSA and blocked with 2% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA = Wheat Germ Agglutinin
ab24186 has been referenced in 4 publications.
- Johnson DC et al. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Nat Med 24:1151-1156 (2018). PubMed: 29967349
- D'Osualdo A et al. CARD8 and NLRP1 undergo autoproteolytic processing through a ZU5-like domain. PLoS One 6:e27396 (2011). WB ; Human . PubMed: 22087307
- Mille F et al. The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 complex. Nat Cell Biol 11:739-46 (2009). WB ; Human . PubMed: 19465923
- Ko DC et al. A genome-wide in vitro bacterial-infection screen reveals human variation in the host response associated with inflammatory disease. Am J Hum Genet 85:214-27 (2009). WB ; Human . PubMed: 19664744