Anti-CARD8 antibody - N-terminal (ab194585)
Key features and details
- Rabbit polyclonal to CARD8 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CARD8 antibody - N-terminal
See all CARD8 primary antibodies -
Description
Rabbit polyclonal to CARD8 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human CARD8 aa 1-200 (N terminal).
Database link: Q9Y2G2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 49 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 49 kDa. |
Target
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Function
Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. -
Tissue specificity
High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow. -
Sequence similarities
Contains 1 CARD domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22900 Human
- Entrez Gene: 100172562 Orangutan
- Omim: 609051 Human
- SwissProt: Q9Y2G2 Human
- SwissProt: Q5RAV7 Orangutan
- Unigene: 446146 Human
- Unigene: 655940 Human
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Alternative names
- Apoptotic protein NDPP 1 antibody
- Apoptotic protein NDPP1 antibody
- CARD 8 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab194585 has been referenced in 5 publications.
- Hsiao JC et al. A ubiquitin-independent proteasome pathway controls activation of the CARD8 inflammasome. J Biol Chem 298:102032 (2022). PubMed: 35580636
- Ball DP et al. Caspase-1 interdomain linker cleavage is required for pyroptosis. Life Sci Alliance 3:N/A (2020). PubMed: 32051255
- Chui AJ et al. Activation of the CARD8 Inflammasome Requires a Disordered Region. Cell Rep 33:108264 (2020). PubMed: 33053349
- Linder A et al. CARD8 inflammasome activation triggers pyroptosis in human T cells. EMBO J 39:e105071 (2020). PubMed: 32840892
- Johnson DC et al. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Nat Med 24:1151-1156 (2018). PubMed: 29967349