Overview

  • Product name
  • Description
    Rabbit polyclonal to CARD9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 36-249 of Human CARD9 (UniProt Q9H257).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and NIH-3T3 cell lysates; Human H1299 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Activates NF-kappa-B via BCL10.
  • Tissue specificity
    Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
  • Involvement in disease
    Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.
  • Sequence similarities
    Contains 1 CARD domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CANDF2 antibody
    • CARD9 antibody
    • CARD9_HUMAN antibody
    • Caspase recruitment domain family member 9 antibody
    • Caspase recruitment domain-containing protein 9 antibody
    • hCARD9 antibody
    see all

Images

  • Anti-CARD9 antibody (ab155166) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 62 kDa



    7.5% SDS PAGE
  • Anti-CARD9 antibody (ab155166) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 62 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded H1299 xenograft tissue labeling CARD9 with ab155166 at 1/100 dilution.

References

ab155166 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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