Overview

  • Product name
    Anti-CARD9 antibody
    See all CARD9 primary antibodies
  • Description
    Mouse polyclonal to CARD9
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human CARD9 (amino acids 1-492; AAH08877.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • CARD9 transfected 293T cell lysate; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Activates NF-kappa-B via BCL10.
  • Tissue specificity
    Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
  • Involvement in disease
    Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.
  • Sequence similarities
    Contains 1 CARD domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • CANDF2 antibody
      • CARD9 antibody
      • CARD9_HUMAN antibody
      • Caspase recruitment domain family member 9 antibody
      • Caspase recruitment domain-containing protein 9 antibody
      • hCARD9 antibody
      see all

    Images

    • Western blot - Anti-CARD9 antibody (ab169623)
      Western blot - Anti-CARD9 antibody (ab169623)
      All lanes : Anti-CARD9 antibody (ab169623) at 1 µg/ml

      Lane 1 : CARD9 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Developed using the ECL technique.

      Predicted band size: 56 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CARD9 antibody (ab169623)
      Immunocytochemistry/ Immunofluorescence - Anti-CARD9 antibody (ab169623)
      Immunofluorescent analysis of HeLa cells labeling CARD9 with ab169623 at 10 µg/ml.

    References

    ab169623 has not yet been referenced specifically in any publications.

    Publishing research using ab169623? Please let us know so that we can cite the reference in this datasheet.

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