Product nameAnti-CARD9 antibody [EPR6489]
See all CARD9 primary antibodies
DescriptionRabbit monoclonal [EPR6489] to CARD9
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CARD9 aa 450-550. The exact sequence is proprietary.
- WB: K562, U937, HL60, HepG2, and THP1 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.12 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 56-62 kDa (predicted molecular weight: 62 kDa).|
FunctionActivates NF-kappa-B via BCL10.
Tissue specificityHighly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
Involvement in diseaseDefects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.
Sequence similaritiesContains 1 CARD domain.
- Information by UniProt
- CANDF2 antibody
- CARD9 antibody
- CARD9_HUMAN antibody
All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/5000 dilution (Purified)
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 52-62 kDa why is the actual band size different from the predicted?
This antibody recognizes 2 isforms
All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/1000 dilution (Unpurified)
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Kan B et al. Cellular metabolism constrains innate immune responses in early human ontogeny. Nat Commun 9:4822 (2018). Read more (PubMed: 30446641) »
- Drummond RA et al. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. PLoS Pathog 11:e1005293 (2015). Flow Cyt ; Human . Read more (PubMed: 26679537) »