Overview

  • Product name

    Anti-CARD9 antibody [EPR6489]
    See all CARD9 primary antibodies
  • Description

    Rabbit monoclonal [EPR6489] to CARD9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CARD9 aa 450-550. The exact sequence is proprietary.

  • Positive control

    • K562, U937, HL60, HepG2, and THP1 cell lysates; HL60 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.12 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6489
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56-62 kDa (predicted molecular weight: 62 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Activates NF-kappa-B via BCL10.
    • Tissue specificity

      Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
    • Involvement in disease

      Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.
    • Sequence similarities

      Contains 1 CARD domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • CANDF2 antibody
      • CARD9 antibody
      • CARD9_HUMAN antibody
      • Caspase recruitment domain family member 9 antibody
      • Caspase recruitment domain-containing protein 9 antibody
      • hCARD9 antibody
      see all

    Images

    • All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : HL60 cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 62 kDa

    • Flow Cytometric analysis of permeabilized HL60 cells labelling CARD9 with ab133560 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Kan B  et al. Cellular metabolism constrains innate immune responses in early human ontogeny. Nat Commun 9:4822 (2018). Read more (PubMed: 30446641) »
    • Drummond RA  et al. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. PLoS Pathog 11:e1005293 (2015). Flow Cyt ; Human . Read more (PubMed: 26679537) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting Abcam regarding ab133560.


    The immunogen sequence used to generate this antibody is as follows: XXX (Please contact us for further details.)


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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