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Our Abpromise guarantee covers the use of ab30807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab30807 staining Cardiac Troponin C in mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with 0.3% Triton in PBS and blocked with 1% BSA + 5% donkey serum for 45 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton + 0.1% BSA + 5% donkey serum) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-goat IgG polyclonal (1/300) was used as the secondary antibody.
Right - goat isotype control.
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