Overview

  • Product name
    Anti-Cardiac Troponin C antibody [EPR9692(B)]
    See all Cardiac Troponin C primary antibodies
  • Description
    Rabbit monoclonal [EPR9692(B)] to Cardiac Troponin C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cardiac Troponin C aa 1-100. The exact sequence is proprietary.

  • Positive control
    • Fetal heart and Human skeletal muscle lysates; Human heart and skeletal muscle tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
    • Involvement in disease
      Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z) [MIM:611879]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13) [MIM:613243]. A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    • Sequence similarities
      Belongs to the troponin C family.
      Contains 4 EF-hand domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • tnnc1a antibody
      • Cardiac troponin C antibody
      • slow skeletal and cardiac muscles antibody
      • TN-C antibody
      • TNC antibody
      • Tnnc1 antibody
      • TNNC1_HUMAN antibody
      • TNNI3 antibody
      • Troponin C antibody
      • Troponin C slow antibody
      • Troponin C1 slow antibody
      see all

    Images

    • All lanes : Anti-Cardiac Troponin C antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labelling Cardiac Troponin C using ab137130 at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling Cardiac Troponin C, using ab137130 at a dilution of 1/100.
    • Immunohistochemical analysis using ab137130 showing positive staining in Heart tissue.

    • Immunohistochemical analysis using ab137130 showing negative staining in Normal brain tissue.

    • Immunohistochemical analysis using ab137130 showing negative staining in Normal kidney tissue.

    • Immunohistochemical analysis using ab137130 showing negative staining in Normal colon tissue.

    • Immunohistochemical analysis using ab137130 showing positive staining in Skeletal muscle tissue.

    References

    This product has been referenced in:
    • Chang H  et al. Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse. J Comp Physiol B 188:863-876 (2018). Read more (PubMed: 30039299) »
    • Chang H  et al. iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus). Proteome Sci 14:16 (2016). WB . Read more (PubMed: 27833457) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137130.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up