RecombinantRabMAb

Recombinant Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)

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Western blot - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
  • Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9692(B)] to Cardiac Troponin C - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free
    See all Cardiac Troponin C primary antibodies

  • Description

    Rabbit monoclonal [EPR9692(B)] to Cardiac Troponin C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Cardiac Troponin C aa 1-100. The exact sequence is proprietary.

  • General notes

    Ab248838 is the carrier-free version of ab137130. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248838 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab248838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function

      Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

    • Involvement in disease

      Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z) [MIM:611879]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13) [MIM:613243]. A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

    • Sequence similarities

      Belongs to the troponin C family.
      Contains 4 EF-hand domains.
    • Information by UniProt

    • Database links

      • Alternative names

        • tnnc1a antibody
        • Cardiac troponin C antibody
        • slow skeletal and cardiac muscles antibody
        • TN-C antibody
        • TNC antibody
        • Tnnc1 antibody
        • TNNC1_HUMAN antibody
        • TNNI3 antibody
        • Troponin C antibody
        • Troponin C slow antibody
        • Troponin C1 slow antibody
        see all

      Images

      • Western blot - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Western blot - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        All lanes : Anti-Cardiac Troponin C antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

        Lane 1 : Fetal heart lysate
        Lane 2 : Human skeletal muscle lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 18 kDa
        Observed band size: 18 kDa



        This data was developed using ab137130, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)

        This data was developed using ab137130, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin embedded Human heart tissue labelling Cardiac Troponin C using ab137130 at a dilution of 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)

        This data was developed using ab137130, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling Cardiac Troponin C, using ab137130 at a dilution of 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        This data was developed using ab137130, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using ab137130 showing positive staining in human Heart tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        This data was developed using ab137130, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using ab137130 showing negative staining in Normal human brain tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        This data was developed using ab137130, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using ab137130 showing negative staining in Normal human kidney tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        This data was developed using ab137130, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using ab137130 showing negative staining in Normal human colon tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        This data was developed using ab137130, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using ab137130 showing positive staining in human Skeletal muscle tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)
        Anti-Cardiac Troponin C antibody [EPR9692(B)] - BSA and Azide free (ab248838)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248838? Please let us know so that we can cite the reference in this datasheet.

      ab248838 has not yet been referenced specifically in any publications.

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