Anti-Cardiac Troponin I antibody [4C2] (ab10231)
- Datasheet
- References (12)
- Protocols
Overview
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Product nameAnti-Cardiac Troponin I antibody [4C2]
See all Cardiac Troponin I primary antibodies -
DescriptionMouse monoclonal [4C2] to Cardiac Troponin I
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Host speciesMouse
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Specificityreacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I.
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Tested applicationsSuitable for: WB, Sandwich ELISA, IHC-P, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Goat, Cow, Cat, Dog, Human, Pig
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Immunogen
Full length protein (Human).
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Epitope23-29 aa
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General notesConcentration varies from lot to lot and can be provided on request.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurity tested by electrophoresis.
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ClonalityMonoclonal
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Clone number4C2
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MyelomaSp2/0
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab10231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| AP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. | |
| Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [19C7] to Cardiac Troponin I (ab92408). Recommended pairs for quantitative sandwich immunoassay: ab10231 (capture), ab92408 (detection) |
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| IHC-P | Use a concentration of 1 µg/ml. | |
| ELISA | Use at an assay dependent concentration. |
Target
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FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
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Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similaritiesBelongs to the troponin I family.
- Information by UniProt
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Database links
- Entrez Gene: 493744 Cat
- Entrez Gene: 511094 Cow
- Entrez Gene: 403566 Dog
- Entrez Gene: 7137 Human
- Entrez Gene: 21954 Mouse
- Entrez Gene: 100049696 Pig
- Entrez Gene: 29248 Rat
- Omim: 191044 Human
see all -
Alternative names
- cardiac muscle antibody
- Cardiac troponin I antibody
- cardiomyopathy, dilated 2A (autosomal recessive) antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)](https://www.abcam.com/ps/products/10/ab10231/Images/Cardiac-Troponin-I-Primary-antibodies-ab10231-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)Ab10231 staining human heart tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References
This product has been referenced in:
- Fu H et al. NIPAAm-MMA nanoparticle-encapsulated visnagin ameliorates myocardial ischemia/reperfusion injury through the promotion of autophagy and the inhibition of apoptosis. Oncol Lett 15:4827-4836 (2018). Read more (PubMed: 29552122) »
- Khodayari Bavil A & Kim J A capillary flow-driven microfluidic system for microparticle-labeled immunoassays. Analyst N/A:N/A (2018). Read more (PubMed: 29878004) »
