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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Troponin
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Anti-Cardiac Troponin I antibody [4C2] (ab10231)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (18)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)

    Key features and details

    • Mouse monoclonal [4C2] to Cardiac Troponin I
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-Cardiac Troponin I antibody [4C2]
      See all Cardiac Troponin I primary antibodies
    • Description

      Mouse monoclonal [4C2] to Cardiac Troponin I
    • Host species

      Mouse
    • Specificity

      reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I.
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length protein (Human).

    • Epitope

      23-29 aa
    • General notes

      Concentration varies from lot to lot and can be provided on request.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purity tested by electrophoresis.
    • Clonality

      Monoclonal
    • Clone number

      4C2
    • Myeloma

      Sp2/0
    • Isotype

      IgG2a
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Troponin
      • Stem Cells
      • Lineage Markers
      • Mesoderm
      • Cardiovascular
      • Heart
      • Contractility
      • Contractile Proteins
      • Troponin
      • Developmental Biology
      • Lineage specification
      • Mesoderm
      • Cardiovascular
      • Cardiovascular Markers
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Cardiomyocytes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human Cardiac Troponin I protein (ab207624)
    • sELISA pair antibody

      • Anti-Cardiac Troponin I antibody [19C7] (ab92408)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10231 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 1 µg/ml.
    Notes
    IHC-P
    Use a concentration of 1 µg/ml.

    Target

    • Function

      Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • Involvement in disease

      Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
      Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Belongs to the troponin I family.
    • Target information above from: UniProt accession P19429 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7137 Human
      • Omim: 191044 Human
      • SwissProt: P19429 Human
      • Unigene: 709179 Human
      • Alternative names

        • cardiac muscle antibody
        • Cardiac troponin I antibody
        • cardiomyopathy, dilated 2A (autosomal recessive) antibody
        • Cardiomyopathy, familial hypertrophic, 7, included antibody
        • CMD1FF antibody
        • CMD2A antibody
        • CMH7 antibody
        • cTnI antibody
        • Familial hypertrophic cardiomyopathy 7 antibody
        • MGC116817 antibody
        • RCM1 antibody
        • Tn1 antibody
        • Tni antibody
        • TNN I3 antibody
        • TNNC 1 antibody
        • TNNC1 antibody
        • TNNI3 antibody
        • TNNI3_HUMAN antibody
        • Troponin I antibody
        • Troponin I cardiac antibody
        • Troponin I cardiac muscle antibody
        • Troponin I cardiac muscle isoform antibody
        • Troponin I type 3 cardiac antibody
        • troponin I, cardiac 3 antibody
        • TroponinI antibody
        • Ttroponin I type 3 (cardiac) antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)
        Ab10231 staining human heart tissue. Staining is localised to cytoplasm.
        Left panel: with primary antibody at 1ug/ml. Right panel: isotype control.
        Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (18)

      Publishing research using ab10231? Please let us know so that we can cite the reference in this datasheet.

      ab10231 has been referenced in 18 publications.

      • Tümer KÇ  et al. Evaluation of cardiac troponin I in serum and myocardium of rabbits with experimentally induced polymicrobial sepsis. Exp Anim 69:54-61 (2020). PubMed: 31462610
      • Sayed N  et al. miRNA transfection via poly(amidoamine)-based delivery vector prevents hypoxia/reperfusion-induced cardiomyocyte apoptosis. Nanomedicine (Lond) 15:163-181 (2020). PubMed: 31799897
      • Cheng Z  et al. SERS-based immunoassay using gold-patterned array chips for rapid and sensitive detection of dual cardiac biomarkers. Analyst 144:6533-6540 (2019). PubMed: 31553332
      • Huang Q  et al. Incremental effect of liraglutide on traditional insulin injections in rats with type 2 diabetes mellitus by maintaining glycolipid metabolism and cardiovascular function. Exp Ther Med 17:1863-1869 (2019). PubMed: 30783461
      • Wall J  et al. Modeling Cardiac Dysfunction Following Traumatic Hemorrhage Injury: Impact on Myocardial Integrity. Front Immunol 10:2774 (2019). PubMed: 31866998
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin I antibody [4C2]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (Cardiomyocyte (heart cell))
      Permeabilization
      Yes - 0.1 % Triton X
      Specification
      Cardiomyocyte (heart cell)
      Blocking step
      Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Ms. Neerajha Nagarajan

      Verified customer

      Submitted Mar 14 2017

      Western blot abreview for Anti-Cardiac Troponin I antibody [4C2]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Cow Tissue lysate - whole (Heart tissue)
      Loading amount
      20 µg
      Specification
      Heart tissue
      Gel Running Conditions
      Reduced Denaturing (12)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 15 2010

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