Key features and details
- Rabbit polyclonal to Cardiac Troponin I
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Pig
- Isotype: IgG
Product nameAnti-Cardiac Troponin I antibody
See all Cardiac Troponin I primary antibodies
DescriptionRabbit polyclonal to Cardiac Troponin I
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Pig
Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Human
Recombinant full length protein (His-T7-tag) corresponding to Pig Cardiac Troponin I aa 2-211. Expressed in E.coli.
ADRSGDAAGDSRPAPAPVRRRSSANYRAYATEPHAKKKSKIPASRKLQLK TLMLQIAKQELEREAEERRGEKGSALSTRCQPLELAGLSFAELQDLCRQL HARVDKVDEERYDVEAKVTKNITEIADLNQKIFDLRGKFKRPTLRRVRIS ADAMMQALLGARAKETLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSG MEGRKKKFEG
Database link: A5X497
- WB: Recombinant pig Cardiac Troponin I; mouse skeletal muscle and heart tissue lysates; pig skeletal muscle and heart lysates. IHC-P : Pig tongue and heart tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography
Our Abpromise guarantee covers the use of ab231064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml.|
FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the troponin I family.
- Information by UniProt
- cardiac muscle antibody
- Cardiac troponin I antibody
- cardiomyopathy, dilated 2A (autosomal recessive) antibody
Formalin-fixed, paraffin-embedded pig heart tissue stained for Cardiac Troponin I with ab231064 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-Cardiac Troponin I antibody (ab231064) at 2 µg/ml + Mouse skeletal muscle lysate
Anti-Cardiac Troponin I antibody (ab231064) at 2 µg/ml + Pig skeletal muscle lysate
Anti-Cardiac Troponin I antibody (ab231064) at 2 mg/ml + Pig heart lysate
Anti-Cardiac Troponin I antibody (ab231064) at 2 µg/ml + Mouse heart lysate
Anti-Cardiac Troponin I antibody (ab231064) at 2 µg/ml + Recombinant pig Cardiac Troponin I
Formalin-fixed, paraffin-embedded pig tongue tissue stained for Cardiac Troponin I with ab231064 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231064 has not yet been referenced specifically in any publications.