Overview

  • Product name
    Anti-Cardiac Troponin I antibody [EPR20307]
    See all Cardiac Troponin I primary antibodies
  • Description
    Rabbit monoclonal [EPR20307] to Cardiac Troponin I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human Cardiac Troponin I aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P19429

  • Positive control
    • WB: Human heart and myocardium lysates; Mouse and rat heart lysates. IHC-P: Human, mouse and rat cardiac muscle tissues. IP: Human fetal heart lysate. IHC-Fr: Mouse heart tissue, Rat heart tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 28 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
IHC-Fr 1/10.

Target

  • Function
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease
    Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities
    Belongs to the troponin I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Images

  • All lanes : Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/2500 dilution

    Lane 1 : Human heart lysate
    Lane 2 : Human myocardium lysate
    Lane 3 : Human skeletal muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression of cardiac Troponin I is restricted to cardiac muscle.

    Negative control:

    Human Skeletal muscle

    PMID: 1934363

    PMID: 8661099

  • Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on human cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemistry (Frozen) analysis of Rat hearttissue section labeling Cardiac Troponin I with purified ab209809 at 1/10 dilution (13.5 µg/ml). Sections were fixed in 0.2% Triton X-100 and permeabilized with DAPI. Antigen retrieval was 4% paraformaldehyde. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/500 dilution (4 µg/ml) dilution. Heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Frozen) analysis of Mouse hearttissue section labeling Cardiac Troponin I with purified ab209809 at 1/10 dilution (13.5 µg/ml). Sections were fixed in 0.2% Triton X-100 and permeabilized with DAPI. Antigen retrieval was 4% paraformaldehyde. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/500 dilution (4 µg/ml) dilution. Heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/5000 dilution + Rat heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/2500 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 3 : Mouse muscle lysate
    Lane 4 : Rat muscle lysate
    Lane 5 : Human brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression of cardiac Troponin I is restricted to cardiac muscle.

    Negative control:

    C2C12, mouse muscle, rat muscle, human brain  

    PMID: 1934363

    PMID: 8661099

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on human skeletal muscle [PMID: 22828728].

    Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on mouse cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on mouse skeletal muscle [PMID: 22828728].

    Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on rat cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on rat skeletal muscle [PMID: 22828728].

    Counter stained with Hematoxylin.

  • Cardiac Troponin I was immunoprecipitated from 0.35 mg of Human fetal heart lysate with ab209809 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209809 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal heart lysate, 10 μg (Input).

    Lane 2: ab209809 IP in Human fetal heart lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209809 in Human fetal heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

ab209809 has not yet been referenced specifically in any publications.

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