Anti-Cardiac Troponin I antibody [M155] (ab10237)
Key features and details
- Mouse monoclonal [M155] to Cardiac Troponin I
- Suitable for: ELISA
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG1
Overview
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Product name
Anti-Cardiac Troponin I antibody [M155]
See all Cardiac Troponin I primary antibodies -
Description
Mouse monoclonal [M155] to Cardiac Troponin I -
Host species
Mouse -
Specificity
This antibody is reacting with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5 mM EDTA). It is not affected by heparin, phosphorylation, oxidation and troponin complex formation. This antibody does not cross-react with skeletal muscle troponin I. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human -
Immunogen
Free human cardiac troponin and/or native troponin complex.
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Epitope
27-43 aa -
General notes
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purity tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
M155 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Target
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Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the troponin I family. - Information by UniProt
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Database links
- Entrez Gene: 403566 Dog
- Entrez Gene: 7137 Human
- Entrez Gene: 21954 Mouse
- Entrez Gene: 29248 Rat
- Omim: 191044 Human
- SwissProt: Q8MKD5 Dog
- SwissProt: P19429 Human
- SwissProt: P48787 Mouse
see all -
Alternative names
- cardiac muscle antibody
- Cardiac troponin I antibody
- cardiomyopathy, dilated 2A (autosomal recessive) antibody
see all
Images
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cTnI calibration curve. Monoclonal antibodies used for capture were biotinylated ab10237 (M155) at 200 ng/well. For detection Eu-labeled ab8289 (19C7) was used at 200 ng/well. The Antigens used included Human (grey triangle), Rat (cream circle), Canine (blue circle), and Mouse (blue square). This was then incubated for 20 min at 20°C.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab10237 has not yet been referenced specifically in any publications.