Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cardiac Troponin I antibody - N-terminal

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% serum in TBS
Concentration
5 µg/ml

Secondary antibody

Concentration
10 µg/ml
Name
Non-Abcam antibody was used: Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondar
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568

Additional data

Additional Notes
This antibody may not work for IHC-P of mouse heart.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.

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Submitted Apr 03 2018

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