Overview

  • Product name
    Anti-Cardiac Troponin T antibody [13-11]
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [13-11] to Cardiac Troponin T
  • Host species
    Mouse
  • Specificity
    Recognizes the cardiac isoform of Troponin T (cTnT). The clone number has been updated from (0.N.590) to (13-11 ) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, IHC-P, Electron Microscopy, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Dog, Human, Pig, Goldfish
    Predicted to work with: Sheep, Cat, Turkey, Zebrafish
  • Immunogen

    Synthetic peptide:

    FMPNLVPPKI

    (5' region of the cardiac gene exon 10),corresponding to Rabbit Cardiac Troponin T

  • Epitope
    TnT4R Epitope.
  • Positive control
    • Recombinant Human Cardiac Troponin T protein (ab86685) can be used as a positive control in WB. Cardiac muscle
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab92546 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab33589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 1 - 2 µg/ml.
Electron Microscopy Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

References

This product has been referenced in:
  • Khaksar M  et al. High glucose condition limited the angiogenic/cardiogenic capacity of murine cardiac progenitor cells in in vitro and in vivo milieu. Cell Biochem Funct N/A:N/A (2018). Read more (PubMed: 30051492) »
  • Vuorenpää H  et al. Maturation of human pluripotent stem cell derived cardiomyocytes is improved in cardiovascular construct. Cytotechnology 69:785-800 (2017). ICC/IF . Read more (PubMed: 28397099) »
See all 13 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us.

The epitope sequence of antibody ab33589 has been updated.

The epitope sequence information of ab998 and ab106076 is proprietary, we unfortunately are not able to disclose this information. These products are guaranteed for applications and species as stated on the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Are you interested in an antibody against a different cardiac marker such as GATA4? The antibody ab86371 looks good.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=86371).

The antibody ab8295, which you used before successfully, is a cardiac troponin T antibody. Do you want another vial of that, to replace the other troponin T antibody, ab33589? If not, then I suggest ab89594.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=89594).

I look forward to your reply.

Read More

Answer

Thank you for sending the sample and protocol details. I think your protocol is good and that the best option now is to replace the antibodies with different antibodies. If you have two in mind, please send the catalogue numbers. If not, I will be happy to make suggestions.

You mentioned that a troponin T antibody gave you good results. Was this a different batch of ab33589, or a different troponin T antibody?

Read More

Answer

Thank you for bringing this to our attention. If I cannot offer a useful suggestion to improve your results, and if the antibodies are covered by our guarantee, I will send replacements.

I will first need a few details of your experiment and your order.

1. Can you please tell me when you ordered the antibodies? If you have a purchase order number or the name of the person who placed the order, that will help me find it in our records.

2. Please describe your samples: what is the species and cell type or tissue, and how were the lysates prepared? Did you use protease inhibitors?

3. How much protein did you load per lane of the gel?

4. Are you confident that the transfer from gel to blot was successful?

5. What is the blocking solution? (For example, 5% milk in TBS or 1% BSA, etc.).

6. You mentioned using the antibodies as advised on the datasheets. What dilutions did you try for each antibody?

7. Are confident the secondary antibody is effective?

I look forward to your reply.

Read More

Question
Answer

Thank you for your call today. The cardiac troponin T antibody ab33589 should recognize the fetal chick isoform, as the immunogen is 100% homologous to a section of the fetal protein based on the SwissProt reference- http://www.uniprot.org/uniprot/P02642 As ab9465 is a monoclonal raised against the full-length purified protein from rabbit, we don't know exactly what region the antibody is targetting so it will be more difficult to predict reactivity. I have emailed the lab to enquire about the type of chick tissue tested with this antibody and I will let you know once I receive a reply. Since it's unknown whether the cell isolation was successful, I would recommend using a positive control (skeletal muscle tissue) to ensure that the antibodies are working in this tissue. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart, ventricular tissue)
Specification
heart, ventricular tissue
Gel Running Conditions
Reduced Denaturing (4~12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

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Verified customer

Submitted Feb 02 2008

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