Overview

  • Product name
    Anti-Cardiac Troponin T antibody [1C11]
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [1C11] to Cardiac Troponin T
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WB, ELISA, IHC-FoFr, IP, IHC-P, Sandwich ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Other Immunogen Type corresponding to Human Cardiac Troponin T aa 171-190.
    Database link: P45379

  • Positive control
    • Natural Human Cardiac Troponin T protein (ab9937) can be used as a positive control in WB. IHC: Human heart tissue. ICC/IF: Cardiomyocytes.
  • General notes

    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

    Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113).

    See other anti-mouse secondary antibodies that can be used with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab8295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 18593559
IHC-Fr Use at an assay dependent concentration. PubMed: 17916803
AP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa. PubMed: 17916803
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19859557
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [9G6] to Cardiac Troponin T (HRP) (ab8296). Can be used as Capture or Detection antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18593559

Target

  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Images

  • Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).

  • ab8295 staining Cardiac Troponin T in mouse cardiac tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.5% Tx100 in PBS and blocked with 1% BSA for 90 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 1% goat serum, 10% BSA + 0.1% Tx100 PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • ab8295 staining Cardiac Troponin T in human pluripotent stem cell derived cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/800) for 1 hour at 21°C. An Alexa Fluor®-568 goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab8295 staining Cardiac Troponin T in the human iPS cell derived from cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% triton X in PBS and blocked with 1% BSA for 18 hours at 4°C. Samples were incubated with primary antibody (1/100 in 1% BSA/PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1 µg/well, detection ab1454 [clone 7E7], 200 ng/well. Assay time, 30 min at room temperature.
  • Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Ren L  et al. A Disintegrin and Metalloprotease-22 Attenuates Hypertrophic Remodeling in Mice Through Inhibition of the Protein Kinase B Signaling Pathway. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29358191) »
  • Shafa M  et al. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers. Front Med (Lausanne) 5:69 (2018). Read more (PubMed: 29600249) »
See all 76 Publications for this product

Customer reviews and Q&As

1-10 of 18 Abreviews or Q&A

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Human Cell (Human embryonic stem cell derived cardiomyocytes)
Specification
Human embryonic stem cell derived cardiomyocytes
Permeabilization
Yes - Triton

Abcam user community

Verified customer

Submitted Dec 16 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 19 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Sample
Human Cell (iPS cell derived cardiomyocyte)
Specification
iPS cell derived cardiomyocyte
Permeabilization
Yes - 0.2% triton X
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 14 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Rat Tissue lysate - other (Left Ventrical, Heart)
Specification
Left Ventrical, Heart
Total protein in input
500 µg

Aaron West

Verified customer

Submitted Feb 18 2014

Answer

Thank you for your e-mail.

Unfortunately we have not determined the pI for ab8295:Anti-Cardiac Troponin T antibody. I am sorry for the inconvenience.

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for contacting us.

Yes, I can confirm the ab105441is the biotinylated form of ab8295, and this antibody shows a good affinity towards the native protein as can be seen in the ELISA and IHC data.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you very much for your patience while I was investigating.


I am happy to confirm that we do have antibody pairs validated for sandwich ELISA for Troponin T, C and I. We do not have tested troponin proteins as standard. For the Troponin T,I have also double checked with the source of the antibodies, and they do have a protein which they can recommend. I am currently checking with my colleagues if we can add it to our catalog. However we do have the individual standard proteins or also a recomplexed protein, which could be tested also as a standard. Please let me know what you think. Indeed, it could be that the recomplexed protein is more stable and less prone to protein degradation and you could use it for all the three ELISAs. potential standard complex: ab94712 https://www.abcam.com/index.html?datasheet=94712 (or use the following: https://www.abcam.com/index.html?datasheet=94712).

Troponin C:
capture antibody: ab8285 https://www.abcam.com/index.html?datasheet=8285 (or use the following: https://www.abcam.com/index.html?datasheet=8285).
detection antibody: ab8286 https://www.abcam.com/index.html?datasheet=8286 (or use the following: https://www.abcam.com/index.html?datasheet=8286).
potential standard protein: ab68911, ab70106 or ab9932 https://www.abcam.com/index.html?datasheet=68911 (or use the following: https://www.abcam.com/index.html?datasheet=68911).

Troponin I:
capture antibody: ab115704 https://www.abcam.com/index.html?datasheet=115704 (or use the following: https://www.abcam.com/index.html?datasheet=115704).
detection antibody: ab19615 https://www.abcam.com/index.html?datasheet=19615 (or use the following: https://www.abcam.com/index.html?datasheet=19615).
potential standard protein: ab50822 https://www.abcam.com/index.html?datasheet=50822 (or use the following: https://www.abcam.com/index.html?datasheet=50822).

Troponin T:
capture antibody: ab9937 https://www.abcam.com/index.html?datasheet=9937 (or use the following: https://www.abcam.com/index.html?datasheet=9937).
detection antibody: ab19615 https://www.abcam.com/index.html?datasheet=19615 (or use the following: https://www.abcam.com/index.html?datasheet=19615).
potential standard protein: ab9937, evt new one https://www.abcam.com/index.html?datasheet=9937 (or use the following: https://www.abcam.com/index.html?datasheet=9937).

For all the proteins or for the complex, we are happy to provide you with our testing discount code as well. Please let me know whether you are interested and I am happy to send you the detailed description of this offer (you would buy the protein to the normal price, test it in ELISA, give us the feedback and then you will get another protein (or other product) for the same price for free. So basically the offer is 100% however only on a second product - for more information, please see also https://www.abcam.com/test-vial)

Unfortunately we do not have any ELISA kits for the moment against the cardiac Troponins. We are however looking into adding them to our catalog within a few months.

I hope this information is helpful. Please do not hesitate to contact me again, should you have any additional questions or should you like to receive the testing discount.

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Answer

Thank you very much for contacting us with your question.

I apologize for the confusion regarding this antibody's storage conditions. We previously recommended storage at 4C for up to one year because that is how the antibody was stored by the lab, and we knew that this temperature was suitable for this product. However, we have recently determined that the antibody can also be stored at -20C, and we recommend this now because theortetically the antibody will be viable for a longer period of time if it is frozen compared to 4C. If you have stored the antibody at 4C, youshould not be concerned, and our Abpromise guarantee still applies to this product.

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

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Answer

Thank you for your email.

The two antibodies will work together. The protein we do not know whether it will work in ELISA.

This is why we could give you a testing discount on the protein. This means, you would buy the protein to the normal price, test it in ELISA, give us the feedback and then you will get another protein (or other product) for the same price for free. So basically the offer is 100% however only on a second product.

The details of the offer can also be found in the last email, where I explained the offer step by step.

Please let me know if this is not clear and if you would wish to clarify some other points.

I hope this is helpful. Please do not hesitate to contact us for any additional information.

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Answer

Thank you very much for your interest in our products.

I am happy to confirm that the ab8295 and the ab8296 are perfectly well choosen together. They will work as a sandwich pair in ELISA.

The ab9937, which you would use as a standard, has not yet been tested in ELISA. It might well work, depending on its conformation. As however we have not assessed experimentally whether the ab9937 can be used in ELISA as a standard, I can offer a discount off a future purchase. If you buyab9937 now, test it inELISA as a standard for ab8295 and ab8296and submit feedback to us in the form of an Abreview, you would receive a discount worht the value of this protein. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab9937 in ELISA. I will then send a discount code. This code must be issued before purchasing ab9937 so please wait for my reply before ordering.

2. Purchase ab9937 either by phone, fax, or online (www.abcam.com).

3. Test it in ELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab9937 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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1-10 of 18 Abreviews or Q&A

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