Anti-Cardiac Troponin T antibody [1C11] (ab8295)

Mouse monoclonal Cardiac Troponin T antibody [1C11]. Validated in WB, IP, ELISA, IHC, Flow Cyt, ICC/IF, sELISA and tested in Mouse, Rat, Dog, Human. Cited in 79 publication(s).

Overview

  • Product name
    Anti-Cardiac Troponin T antibody [1C11]
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [1C11] to Cardiac Troponin T
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WB, ELISA, IHC-FoFr, IP, IHC-P, Sandwich ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Other Immunogen Type corresponding to Human Cardiac Troponin T aa 171-190.
    Database link: P45379

  • Positive control
    • Natural Human Cardiac Troponin T protein (ab9937) can be used as a positive control in WB. IHC: Human heart tissue. ICC/IF: Cardiomyocytes.
  • General notes

    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

    Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113).

    See other anti-mouse secondary antibodies that can be used with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab8295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 18593559
IHC-Fr Use at an assay dependent concentration. PubMed: 17916803
AP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa. PubMed: 17916803
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19859557
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use at an assay dependent concentration. Can be used as Capture or Detection antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18593559

Target

  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Images

  • Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).

  • ab8295 staining Cardiac Troponin T in mouse cardiac tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.5% Tx100 in PBS and blocked with 1% BSA for 90 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 1% goat serum, 10% BSA + 0.1% Tx100 PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • ab8295 staining Cardiac Troponin T in human pluripotent stem cell derived cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/800) for 1 hour at 21°C. An Alexa Fluor®-568 goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab8295 staining Cardiac Troponin T in the human iPS cell derived from cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% triton X in PBS and blocked with 1% BSA for 18 hours at 4°C. Samples were incubated with primary antibody (1/100 in 1% BSA/PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1 µg/well, detection ab1454 [clone 7E7], 200 ng/well. Assay time, 30 min at room temperature.
  • Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Zhang J  et al. Insulin gene enhancer binding protein 1 induces adipose tissue-derived stem cells to differentiate into pacemaker-like cells. Int J Mol Med 43:879-889 (2019). Read more (PubMed: 30483766) »
  • Li S  et al. Fibroblast growth factor 21 protects the heart from angiotensin II-induced cardiac hypertrophy and dysfunction via SIRT1. Biochim Biophys Acta Mol Basis Dis N/A:N/A (2019). Read more (PubMed: 30677512) »
See all 84 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-6 of 6 Abreviews

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Human Cell (Human embryonic stem cell derived cardiomyocytes)
Specification
Human embryonic stem cell derived cardiomyocytes
Permeabilization
Yes - Triton

Abcam user community

Verified customer

Submitted Dec 16 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 19 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Sample
Human Cell (iPS cell derived cardiomyocyte)
Specification
iPS cell derived cardiomyocyte
Permeabilization
Yes - 0.2% triton X
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 14 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Rat Tissue lysate - other (Left Ventrical, Heart)
Specification
Left Ventrical, Heart
Total protein in input
500 µg

Aaron West

Verified customer

Submitted Feb 18 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Tx100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 18 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (heart, ventricular tissue)
Loading amount
60 µg
Specification
heart, ventricular tissue
Gel Running Conditions
Reduced Denaturing (4~12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 02 2008

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