Anti-Cardiac Troponin T antibody [1F11] (ab10214)
![Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)](https://www.abcam.com/ps/products/10/ab10214/Images/ab10214-6-anti-cardiac-troponin-t-immunocytochemistry-antibody-ips-myocytes-human-1f11.jpg "Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)")
Key features and details
- Mouse monoclonal [1F11] to Cardiac Troponin T
- Suitable for: Sandwich ELISA, ICC
- Reacts with: Rabbit, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-Cardiac Troponin T antibody [1F11]
See all Cardiac Troponin T primary antibodies -
Description
Mouse monoclonal [1F11] to Cardiac Troponin T -
Host species
Mouse -
Specificity
Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813) -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIP Mouse
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Immunogen
Free human cardiac troponin T
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Epitope
Peptide 146-160. -
Positive control
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)
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General notes
This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity tested by electrophoresis. -
Primary antibody notes
Does not cross-react with skeletal muscle troponin T, cTnI or TnC. -
Clonality
Monoclonal -
Clone number
1F11 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
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| IP |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
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| ICC | (2) |
Use a concentration of 1 - 5 µg/ml.
|
| Notes |
|---|
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Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection. |
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ICC
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Tissue specificity
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
Involvement in disease
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
Sequence similarities
Belongs to the troponin T family. - Information by UniProt
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Database links
- Entrez Gene: 100009428 Rabbit
- SwissProt: P09741 Rabbit
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Alternative names
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
Images
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Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
Immunofluorescence staining of Cardiac Troponin T using ab10214 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab10214 at 1 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab10214 also gave a positive staining using 4% formaldehyde fixation (10 min).
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![Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)](https://www.abcam.com/ps/products/10/ab10214/Images/ab10214-17783-anti-cardiac-troponin-t-antibody-1f11-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab10214 has been referenced in 39 publications.
- Garbutt TA et al. An Optimized Protocol for Human Direct Cardiac Reprogramming. STAR Protoc 1:N/A (2020). PubMed: 32728671
- Bae JH et al. ZNF746/PARIS overexpression induces cellular senescence through FoxO1/p21 axis activation in myoblasts. Cell Death Dis 11:359 (2020). PubMed: 32398756
- Zhou Y et al. Single-Cell Transcriptomic Analyses of Cell Fate Transitions during Human Cardiac Reprogramming. Cell Stem Cell 25:149-164.e9 (2019). PubMed: 31230860
- Iismaa SE et al. Cardiac hypertrophy limits infarct expansion after myocardial infarction in mice. Sci Rep 8:6114 (2018). PubMed: 29666426
- Chaturvedi P et al. Stable and reproducible transgene expression independent of proliferative or differentiated state using BAC TG-EMBED. Gene Ther N/A:N/A (2018). PubMed: 29930343