Anti-Cardiac Troponin T antibody [1F11] (ab10214)

Reviews (5)Q&A (5)References (33)

Overview

  • Product name
    Anti-Cardiac Troponin T antibody [1F11]
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [1F11] to Cardiac Troponin T
  • Host species
    Mouse
  • Specificity
    Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813)
  • Tested applications
    Suitable for: Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, Other, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Fish
  • Immunogen

    Free human cardiac troponin T

  • Epitope
    Peptide 146-160.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Concentration varies from lot to lot and can be provided on request.

    Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Properties

Applications

Our Abpromise guarantee covers the use of ab10214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21278734

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
ICC Use at an assay dependent concentration. Used at a dilution of 1/400 for 1 hr incubation on rat cardiomyocytes (see Abreview for further details).
Other Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 23066088

Target

  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
    Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [1F11] (ab10214)This image is courtesy of an anonymous Abreview

    ab10214 staining human spontaneous differentiating iPSC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.25%% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
    Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
    Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
  • Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
    Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)

    ab10214 at a 1/400 dilution staining rat cardiomyocytes by immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 2% serum. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using an Alexa Fluor® 594 conjugated donkey Anti-Mouse IgG(H+L).

    This image is courtesy of an Abreview submitted by Yiqiang Zhang.

    See Abreview

References

This product has been referenced in:
  • Iismaa SE  et al. Cardiac hypertrophy limits infarct expansion after myocardial infarction in mice. Sci Rep 8:6114 (2018). Read more (PubMed: 29666426) »
  • Chaturvedi P  et al. Stable and reproducible transgene expression independent of proliferative or differentiated state using BAC TG-EMBED. Gene Ther N/A:N/A (2018). Read more (PubMed: 29930343) »
See all 33 Publications for this product

Publishing research using ab10214? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1F11]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (spontaneous differentiating iPSC)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
spontaneous differentiating iPSC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 10 2016

Immunoprecipitation abreview for Anti-Cardiac Troponin T antibody [1F11]

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Cardiac cells)
Total protein in input
50 µg
Immuno-precipitation step
Protein A/G
Specification
Cardiac cells
Read More

Abcam user community

Verified customer

Submitted Jun 27 2015

Question

Dear All,

We receive yesterday item AB10214 lot GR84414-2 orderd with PO# 1875 (Nov 29th).

I inform you the vial was not tight enough, and the antibody volume was almost zero.

Can you please authorize a new vial as free of charge replacement?

Best regards,

Read More
Answer

Thank you for your contact. I am very sorry to hear that the vial was empty.
This is to let you know that I have placed a new order as a free of charge replacement of ab10214. The new order number is 1217998.
If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Question

We have made several publications with your antibody ab10214 measuring
Troponin with Surface Plasmon Resonanse (SPR). Now we are trying to
continue the work with a new sensor technology. However we are unable to
get the system to work. As one control we have repeated previous
published experiments on SPR without success. Is there any known issues
on this Lot No: GR28930? Do you have any suggestions for another
antibody that may work better? Is it possible to get another sample of
this ab on the Abpromise guarantee? I have spent a lot of time
troubleshooting and now I'm out of ideas.

Read More
Answer

Thank you for contacting us.
I am sorry to hear that the antibody that worked previously is not working anymore.

I checked our records for this lot number, but have not found any complaints. We do not test any antibodies in SPR, thus in general would not guarantee this application.

In this case, however, I'd be happy to make an one-time exception, and send you another lot free of charge. Please provide me also with the following information:

1) Could you please send me more details about how the antibody is being used (dilution, incubation time, samples, species, etc.).

2) How is the new sensor technology different from the old one in terms of antibody usage?

3) I would suggest - if possible - to use the antibody on a tested application (besides SPR; such as Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, ICC/IF) to check if it works in one of these as expected.

4) If you could send me your publication links, I'd be happy to have them added to the antibody datasheet - showing that the antibody can work in SPR.

I look forward to hear back from you and assist you further.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question

I bought from abcam the ref # ab10214 antibody. I used it to check Troponin T in the mice heart tissue. I sow three bands by western blot (attached Power point). Could you tell me what correspond this bands?

Read More
Answer

Thank you for contacting us and for providing this image.

After review of the image provided, we believe that the extra bands which you are seeing are result of non-specific background. This will show up when using too much protein, too much antibody, an ECL reagent which is extremely sensitive or when exposing for too long. We recommend reducing the amount of protein added per well and the concentration of the antibody used. We also recommend exposing your blot for a shorter period of time, perhaps one minute of 30 seconds.

I’d like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMAb free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Please give me a new code for ab86371 and dog. Thank you for your help!

Best wishes for you!

Read More
Answer



I could certainly offer you a code number. Would you be so kind to confirm the name and the address of your research institute? We need this information only for our internal record.

Thank you and I look forward to hearing from you soon.

Read More

Question

Dear,

I am a Phd student at the KU Leuven, Belgium.

I am planning to order 3 antibodies (ab10214, ab15, ab119952) from your catalogous to perform flow cytometry with.
These antibodies are made in mouse but I work with murine embryonic stem cells. The data sheet of the 3 antibodies are showing reactivity with mouse.
Can I use it for my murine cells? How can I avoid aspecific binding?

Many thanks in advance!

Best regards,

Read More
Answer

Thank you for your enquiry and your interest in our products.

I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal problem mainly in immunohistochemical using tissue sections (mouse-on-mouse) due to endogenous mouse immunoglobins. In order to be able to reduce the risk of any high background signal, you may wish to select Fab fragment secondary antibodies - rather than full molecules.

I would recommend visiting this website; You may find some useful information on how to block high background signal:

https://www.abcam.com/index.html?pageconfig=resource&rid=11465

https://docs.abcam.com/pdf/protocols/Mouse-on-Mouse-Protocol.pdf

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Western blot abreview for Anti-Cardiac Troponin T antibody [1F11]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Cow Tissue lysate - whole (heart tissue)
Loading amount
20 µg
Specification
heart tissue
Gel Running Conditions
Reduced Denaturing (13)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Feb 15 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1F11]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (ES cell EBs differentiated for 12 days.)
Specification
ES cell EBs differentiated for 12 days.
Fixative
Methanol
Permeabilization
Yes - 0.1%triton-x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Apr 22 2008

Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1F11]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunocytochemistry
Sample
Rat Cell (cardiomyocytes)
Specification
cardiomyocytes
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Read More

Dr. Yiqiang Zhang

Verified customer

Submitted Jun 09 2006

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