Anti-Cardiac Troponin T antibody [1F11] (ab10214)
- Datasheet
- Specific References (30)
- Protocols
Overview
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Product nameAnti-Cardiac Troponin T antibody [1F11]
See all Cardiac Troponin T primary antibodies -
DescriptionMouse monoclonal [1F11] to Cardiac Troponin T
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Host speciesMouse
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SpecificitySuitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813)
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Tested applicationsSuitable for: Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, Other, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Rat, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Fish
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Immunogen
Free human cardiac troponin T
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EpitopePeptide 146-160.
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General notes
This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurity tested by electrophoresis.
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Primary antibody notesDoes not cross-react with skeletal muscle troponin T, cTnI or TnC.
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ClonalityMonoclonal
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Clone number1F11
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MyelomaSp2/0
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Related Products
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab10214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. PubMed: 21278734 ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| AP | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa. | |
| Sandwich ELISA | Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection. | |
| ICC | Use at an assay dependent concentration. Used at a dilution of 1/400 for 1 hr incubation on rat cardiomyocytes (see Abreview for further details). | |
| Other | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 23066088 |
Target
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FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
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Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
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Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
Sequence similaritiesBelongs to the troponin T family.
- Information by UniProt
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Database links
- Entrez Gene: 493940 Cat
- Entrez Gene: 286816 Cow
- Entrez Gene: 403532 Dog
- Entrez Gene: 7139 Human
- Entrez Gene: 100622450 Pig
- Entrez Gene: 100009428 Rabbit
- Entrez Gene: 24837 Rat
- Omim: 191045 Human
see all -
Alternative names
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
Images
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![Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)](https://www.abcam.com/ps/products/10/ab10214/Images/Cardiac-Troponin-T-Primary-antibodies-ab10214-4.jpg)
Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml. -
![Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [1F11] (ab10214)](https://www.abcam.com/ps/products/10/ab10214/Images/ab10214-262199-troponin.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [1F11] (ab10214)This image is courtesy of an anonymous Abreviewab10214 staining human spontaneous differentiating iPSC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.25%% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
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![Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)](https://www.abcam.com/ps/products/10/ab10214/Images/ab10214_1.jpg)
Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)ab10214 at a 1/400 dilution staining rat cardiomyocytes by immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 2% serum. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using an Alexa Fluor® 594 conjugated donkey Anti-Mouse IgG(H+L).
This image is courtesy of an Abreview submitted by Yiqiang Zhang.
Protocols
References
This product has been referenced in:
- Iismaa SE et al. Cardiac hypertrophy limits infarct expansion after myocardial infarction in mice. Sci Rep 8:6114 (2018). Read more (PubMed: 29666426) »
- Binek A et al. Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model. Sci Rep 7:12343 (2017). WB ; Pig . Read more (PubMed: 28955040) »
Customer reviews and Q&As
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