Anti-Cardiac Troponin T antibody [1F11] (ab10214)

Reviews (5)Q&A (5)References (49)
Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
  • Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)

Key features and details

  • Mouse monoclonal [1F11] to Cardiac Troponin T
  • Suitable for: Sandwich ELISA, ICC
  • Reacts with: Rabbit, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Cardiac Troponin T antibody [1F11]
    See all Cardiac Troponin T primary antibodies

  • Description

    Mouse monoclonal [1F11] to Cardiac Troponin T

  • Host species

    Mouse

  • Specificity

    Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813)

  • Tested applications

    Suitable for: Sandwich ELISA, ICCmore details

  • Species reactivity

    Reacts with: Rabbit, Recombinant fragment

  • Immunogen

    Tissue, cells or virus corresponding to Human Cardiac Troponin T. Free human cardiac troponin T

  • Epitope

    Peptide 146-160.

  • Positive control

    • ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)

  • General notes

    This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Concentration varies from lot to lot and can be provided on request.

    Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
ICC (2)
Use a concentration of 1 - 5 µg/ml.
Notes
Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
ICC
Use a concentration of 1 - 5 µg/ml.

Target

  • Function

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

  • Tissue specificity

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

  • Involvement in disease

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.

  • Sequence similarities

    Belongs to the troponin T family.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    Images

    • Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
      Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)

      Immunofluorescence staining of Cardiac Troponin T using ab10214 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.


      The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab10214 at 1 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.


      The antibody ab10214 also gave a positive staining using 4% formaldehyde fixation (10 min).

    • Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
      Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
      Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.

    References (49)

    Publishing research using ab10214? Please let us know so that we can cite the reference in this datasheet.

    ab10214 has been referenced in 49 publications.

    • Li Y  et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs. Sci Adv 7:N/A (2021). PubMed: 33627421
    • Wang Q  et al. Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells. Sci China Life Sci 64:269-281 (2021). PubMed: 32712833
    • Ntege EH  et al. Effects of hydroxyapatite-coated nonwoven polyethylene/polypropylene fabric on non-mesodermal lineage-specific differentiation of human adipose-derived stem cells. BMC Res Notes 13:471 (2020). PubMed: 33028399
    • Alam MJ  et al. Catestatin reverses the hypertrophic effects of norepinephrine in H9c2 cardiac myoblasts by modulating the adrenergic signaling. Mol Cell Biochem 464:205-219 (2020). PubMed: 31792650
    • Fan C  et al. Myocardial protection by nanomaterials formulated with CHIR99021 and FGF1. JCI Insight 5:N/A (2020). PubMed: 32453715
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1F11]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (spontaneous differentiating iPSC)
    Permeabilization
    Yes - 0.25% Triton X-100 in PBS
    Specification
    spontaneous differentiating iPSC
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    Immunoprecipitation abreview for Anti-Cardiac Troponin T antibody [1F11]

     Good
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Cardiac cells)
    Total protein in input
    50 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    Cardiac cells
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 27 2015

    Western blot abreview for Anti-Cardiac Troponin T antibody [1F11]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (heart tissue)
    Loading amount
    20 µg
    Specification
    heart tissue
    Gel Running Conditions
    Reduced Denaturing (13)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 15 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1F11]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (ES cell EBs differentiated for 12 days.)
    Specification
    ES cell EBs differentiated for 12 days.
    Fixative
    Methanol
    Permeabilization
    Yes - 0.1%triton-x
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 22 2008

    Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1F11]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rat Cell (cardiomyocytes)
    Specification
    cardiomyocytes
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
    Read More

    Dr. Yiqiang Zhang

    Verified customer

    Submitted Jun 09 2006

    Question

    Dear All,

    We receive yesterday item AB10214 lot GR84414-2 orderd with PO# 1875 (Nov 29th).

    I inform you the vial was not tight enough, and the antibody volume was almost zero.

    Can you please authorize a new vial as free of charge replacement?

    Best regards,

    Read More
    Answer

    Thank you for your contact. I am very sorry to hear that the vial was empty.
    This is to let you know that I have placed a new order as a free of charge replacement of ab10214. The new order number is 1217998.
    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    We have made several publications with your antibody ab10214 measuring
    Troponin with Surface Plasmon Resonanse (SPR). Now we are trying to
    continue the work with a new sensor technology. However we are unable to
    get the system to work. As one control we have repeated previous
    published experiments on SPR without success. Is there any known issues
    on this Lot No: GR28930? Do you have any suggestions for another
    antibody that may work better? Is it possible to get another sample of
    this ab on the Abpromise guarantee? I have spent a lot of time
    troubleshooting and now I'm out of ideas.

    Read More
    Answer

    Thank you for contacting us.
    I am sorry to hear that the antibody that worked previously is not working anymore.

    I checked our records for this lot number, but have not found any complaints. We do not test any antibodies in SPR, thus in general would not guarantee this application.

    In this case, however, I'd be happy to make an one-time exception, and send you another lot free of charge. Please provide me also with the following information:

    1) Could you please send me more details about how the antibody is being used (dilution, incubation time, samples, species, etc.).

    2) How is the new sensor technology different from the old one in terms of antibody usage?

    3) I would suggest - if possible - to use the antibody on a tested application (besides SPR; such as Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, ICC/IF) to check if it works in one of these as expected.

    4) If you could send me your publication links, I'd be happy to have them added to the antibody datasheet - showing that the antibody can work in SPR.

    I look forward to hear back from you and assist you further.


    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    I bought from abcam the ref # ab10214 antibody. I used it to check Troponin T in the mice heart tissue. I sow three bands by western blot (attached Power point). Could you tell me what correspond this bands?

    Read More
    Answer

    Thank you for contacting us and for providing this image.

    After review of the image provided, we believe that the extra bands which you are seeing are result of non-specific background. This will show up when using too much protein, too much antibody, an ECL reagent which is extremely sensitive or when exposing for too long. We recommend reducing the amount of protein added per well and the concentration of the antibody used. We also recommend exposing your blot for a shorter period of time, perhaps one minute of 30 seconds.

    I’d like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMAb free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Please give me a new code for ab86371 and dog. Thank you for your help!

    Best wishes for you!

    Read More
    Answer



    I could certainly offer you a code number. Would you be so kind to confirm the name and the address of your research institute? We need this information only for our internal record.

    Thank you and I look forward to hearing from you soon.

    Read More

    Question

    Dear,

    I am a Phd student at the KU Leuven, Belgium.

    I am planning to order 3 antibodies (ab10214, ab15, ab119952) from your catalogous to perform flow cytometry with.
    These antibodies are made in mouse but I work with murine embryonic stem cells. The data sheet of the 3 antibodies are showing reactivity with mouse.
    Can I use it for my murine cells? How can I avoid aspecific binding?

    Many thanks in advance!

    Best regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal problem mainly in immunohistochemical using tissue sections (mouse-on-mouse) due to endogenous mouse immunoglobins. In order to be able to reduce the risk of any high background signal, you may wish to select Fab fragment secondary antibodies - rather than full molecules.

    I would recommend visiting this website; You may find some useful information on how to block high background signal:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11465

    https://docs.abcam.com/pdf/protocols/Mouse-on-Mouse-Protocol.pdf

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com