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Free human cardiac troponin T
This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
Our Abpromise guarantee covers the use of ab10214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21278734
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|AP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.|
|ICC||Use at an assay dependent concentration. Used at a dilution of 1/400 for 1 hr incubation on rat cardiomyocytes (see Abreview for further details).|
|Other||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 23066088|
ab10214 staining human spontaneous differentiating iPSC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.25%% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
ab10214 at a 1/400 dilution staining rat cardiomyocytes by immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 2% serum. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using an Alexa Fluor® 594 conjugated donkey Anti-Mouse IgG(H+L).
This image is courtesy of an Abreview submitted by Yiqiang Zhang.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"