• Product name
    Anti-Cardiac Troponin T antibody [1F11]
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [1F11] to Cardiac Troponin T
  • Host species
  • Specificity
    Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813)
  • Tested applications
    Suitable for: Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, Other, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Fish
  • Immunogen

    Free human cardiac troponin T

  • Epitope
    Peptide 146-160.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Concentration varies from lot to lot and can be provided on request.

    Does not cross-react with skeletal muscle troponin T, cTnI or TnC.



Our Abpromise guarantee covers the use of ab10214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21278734

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
ICC Use at an assay dependent concentration. Used at a dilution of 1/400 for 1 hr incubation on rat cardiomyocytes (see Abreview for further details).
Other Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 23066088


  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all


  • ab10214 staining human spontaneous differentiating iPSC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.25%% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
  • ab10214 at a 1/400 dilution staining rat cardiomyocytes by immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 2% serum. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using an Alexa Fluor® 594 conjugated donkey Anti-Mouse IgG(H+L).

    This image is courtesy of an Abreview submitted by Yiqiang Zhang.

    See Abreview


This product has been referenced in:
  • Iismaa SE  et al. Cardiac hypertrophy limits infarct expansion after myocardial infarction in mice. Sci Rep 8:6114 (2018). Read more (PubMed: 29666426) »
  • Chaturvedi P  et al. Stable and reproducible transgene expression independent of proliferative or differentiated state using BAC TG-EMBED. Gene Ther N/A:N/A (2018). Read more (PubMed: 29930343) »
See all 33 Publications for this product

Customer reviews and Q&As

1-5 of 5 Q&A


Thank you for your contact. I am very sorry to hear that the vial was empty.
This is to let you know that I have placed a new order as a free of charge replacement of ab10214. The new order number is 1217998.
If you need any further assistance in the future, please do not hesitate to contact me.

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Thank you for contacting us.
I am sorry to hear that the antibody that worked previously is not working anymore.

I checked our records for this lot number, but have not found any complaints. We do not test any antibodies in SPR, thus in general would not guarantee this application.

In this case, however, I'd be happy to make an one-time exception, and send you another lot free of charge. Please provide me also with the following information:

1) Could you please send me more details about how the antibody is being used (dilution, incubation time, samples, species, etc.).

2) How is the new sensor technology different from the old one in terms of antibody usage?

3) I would suggest - if possible - to use the antibody on a tested application (besides SPR; such as Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, ICC/IF) to check if it works in one of these as expected.

4) If you could send me your publication links, I'd be happy to have them added to the antibody datasheet - showing that the antibody can work in SPR.

I look forward to hear back from you and assist you further.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Thank you for contacting us and for providing this image.

After review of the image provided, we believe that the extra bands which you are seeing are result of non-specific background. This will show up when using too much protein, too much antibody, an ECL reagent which is extremely sensitive or when exposing for too long. We recommend reducing the amount of protein added per well and the concentration of the antibody used. We also recommend exposing your blot for a shorter period of time, perhaps one minute of 30 seconds.

I’d like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMAb free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

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I could certainly offer you a code number. Would you be so kind to confirm the name and the address of your research institute? We need this information only for our internal record.

Thank you and I look forward to hearing from you soon.

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Thank you for your enquiry and your interest in our products.

I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal problem mainly in immunohistochemical using tissue sections (mouse-on-mouse) due to endogenous mouse immunoglobins. In order to be able to reduce the risk of any high background signal, you may wish to select Fab fragment secondary antibodies - rather than full molecules.

I would recommend visiting this website; You may find some useful information on how to block high background signal:



I hope this helps and if I can assist further, please do not hesitate to contact me.

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