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  1. Link

    cardiac-troponin-t-antibody-ab115134.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Troponin
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Anti-Cardiac Troponin T antibody (ab115134)

  • Datasheet
Submit a review Submit a question References (3)

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Western blot - Anti-Cardiac Troponin T antibody (ab115134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab115134)

Key features and details

  • Rabbit polyclonal to Cardiac Troponin T
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Cardiac Troponin T antibody
    See all Cardiac Troponin T primary antibodies
  • Description

    Rabbit polyclonal to Cardiac Troponin T
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Heart; Rat Heart; Human Fetal Heart. It also gave a positive result in FFPE mouse heart tissue sections.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Cardiomyocytes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cardiac Troponin T protein (ab86685)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab115134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).

Target

  • Function

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities

    Belongs to the troponin T family.
  • Target information above from: UniProt accession P45379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7139 Human
    • Entrez Gene: 21956 Mouse
    • Entrez Gene: 24837 Rat
    • Omim: 191045 Human
    • SwissProt: P45379 Human
    • SwissProt: P50752 Mouse
    • SwissProt: P50753 Rat
    • Unigene: 533613 Human
    • Unigene: 247470 Mouse
    • Unigene: 9965 Rat
    see all
  • Alternative names

    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Images

  • Western blot - Anti-Cardiac Troponin T antibody (ab115134)
    Western blot - Anti-Cardiac Troponin T antibody (ab115134)
    All lanes : Anti-Cardiac Troponin T antibody (ab115134) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate
    Lane 3 : Heart (Human) Whole Cell Lysate - fetal normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?
    Additional bands at: 29 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab115134)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab115134)
    IHC image of Cardiac Troponin T staining in mouse heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115134, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (3)

Publishing research using ab115134? Please let us know so that we can cite the reference in this datasheet.

ab115134 has been referenced in 3 publications.

  • Kwak HJ  et al. Behavioral variation according to feeding organ diversification in glossiphoniid leeches (Phylum: Annelida). Sci Rep 11:10940 (2021). PubMed: 34035418
  • Li K  et al. Tanshinone IIA promotes cardiac differentiation and improves cell motility by modulating the Wnt/ß-catenin signaling pathway. Mol Med Rep 22:1839-1846 (2020). PubMed: 32582982
  • Thiele F  et al. Nkx2.5 Based Ventricular Programming of Murine ESC-Derived Cardiomyocytes. Cell Physiol Biochem 53:337-354 (2019). PubMed: 31373783

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