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    cardiac-troponin-t-antibody-ab45932.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Troponin
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Anti-Cardiac Troponin T antibody (ab45932)

  • Datasheet
  • SDS
Reviews (7)Q&A (6)References (42)

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Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody (ab45932)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab45932)
  • Western blot - Anti-Cardiac Troponin T antibody (ab45932)
  • Sandwich ELISA - Anti-Cardiac Troponin T antibody (ab45932)

Key features and details

  • Rabbit polyclonal to Cardiac Troponin T
  • Suitable for: IHC-P, Sandwich ELISA, WB
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human Cardiac Troponin T protein (ab86685)

View more associated products

Overview

  • Product name

    Anti-Cardiac Troponin T antibody
    See all Cardiac Troponin T primary antibodies
  • Description

    Rabbit polyclonal to Cardiac Troponin T
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    sELISA
    Recombinant fragment
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human cardiac Troponin T.

    Read Abcam's proprietary immunogen policy (Peptide available as ab47005.)
  • General notes

      

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin
    • Cardiovascular
    • Cardiovascular Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Cardiomyocytes
    • Signal Transduction
    • Antibodies
    • cardiac troponin t

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cardiac Troponin T protein (ab86685)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Recombinant Human Cardiac Troponin T protein (ab86685)
  • sELISA pair antibody

    • Anti-Cardiac Troponin T antibody [1F11] (ab10214)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
sELISA
Recombinant fragment
WB
Human
All applications
Rat
Rabbit
Guinea pig
Cow
Cat
Dog
Application Abreviews Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) as Capture.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) as Capture.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).

Target

  • Function

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities

    Belongs to the troponin T family.
  • Target information above from: UniProt accession P45379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 493940 Cat
    • Entrez Gene: 286816 Cow
    • Entrez Gene: 403532 Dog
    • Entrez Gene: 7139 Human
    • Entrez Gene: 100009428 Rabbit
    • Entrez Gene: 24837 Rat
    • Omim: 191045 Human
    • SwissProt: P13789 Cow
    • SwissProt: P45379 Human
    • SwissProt: P09741 Rabbit
    • SwissProt: P50753 Rat
    • Unigene: 533613 Human
    • Unigene: 9965 Rat
    see all
  • Alternative names

    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody (ab45932)
    Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody (ab45932)This image is courtesy of an Abreview submitted by Tova Volberg

    ab45932 staining Cardiac Troponin T in rat cardiac cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in 3% paraformaldehyde. Samples were incubated with primary antibody (1/400 in diluent) for 1 hour at room temperature. A Cy3®-conjugated goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab45932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab45932)
    IHC image of Cardiac Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45932, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot - Anti-Cardiac Troponin T antibody (ab45932)
    Western blot - Anti-Cardiac Troponin T antibody (ab45932)
    Anti-Cardiac Troponin T antibody (ab45932) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Sandwich ELISA - Anti-Cardiac Troponin T antibody (ab45932)
    Sandwich ELISA - Anti-Cardiac Troponin T antibody (ab45932)
    Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (42)

    Publishing research using ab45932? Please let us know so that we can cite the reference in this datasheet.

    ab45932 has been referenced in 42 publications.

    • Homma J  et al. A novel method to align cells in a cardiac tissue-like construct fabricated by cell sheet-based tissue engineering. J Tissue Eng Regen Med N/A:N/A (2020). PubMed: 32478904
    • Meng X  et al. Fetal Hypoxia Impacts on Proliferation and Differentiation of Sca-1+ Cardiac Progenitor Cells and Maturation of Cardiomyocytes: A Role of MicroRNA-210. Genes (Basel) 11:N/A (2020). PubMed: 32244901
    • Zhang JZ  et al. A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. Cell Stem Cell 24:802-811.e5 (2019). PubMed: 30880024
    • Hamad S  et al. Generation of human induced pluripotent stem cell-derived cardiomyocytes in 2D monolayer and scalable 3D suspension bioreactor cultures with reduced batch-to-batch variations. Theranostics 9:7222-7238 (2019). PubMed: 31695764
    • Acun A  et al. In vitro aged, hiPSC-origin engineered heart tissue models with age-dependent functional deterioration to study myocardial infarction. Acta Biomater 94:372-391 (2019). PubMed: 31146032
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    Question

    Our customer wishes to test ab45932 in ICC/IF with rat samples.
    Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)? If your answer is positive Thanks in advance for your reply.
    Have a nice day.

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2012

    Answer

    DISCOUNT CODE: *******
    Expiration date: DD MM YYYY
    I am very pleased to hear you would like to accept our offer and test ab45932 in Rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on May 03 2012

    Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (differentiated iPSC)
    Specification
    differentiated iPSC
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 12 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Specification
    Human pluripotent stem cell derived cardiomyocyte
    Permeabilization
    Yes - saponin
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 19 2014

    Question

    Give any reference in which this antibody recognise both intact and proteolysis fragments of cardiac troponin -T on western blot.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    Thank you for your enquiry. We are not aware of any references for the antibody ab45932 that include western blot data demonstrating recognition of intact and fragmented troponin T. There is a western blot of human heart tissue on the datasheet which shows detection of a band at about 40 kDa, in addition to several smaller, less intense bands which may represent isoforms of troponin T.

    Read More

    Abcam Scientific Support

    Answered on Oct 22 2012

    Question

    Is abcam 45932 recognise epitope located between 50-150 amino acid residue. Is this product identify fragments of cardiac troponin-T

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    Thank you for contacting us.

    The antibody ab45932 recognizes the amino acid sequence of the immunogen, so it should be capable of detecting protein fragments that contain this sequence, using the applications listed on the datasheet: western blotting, ELISA, etc..

    All of the isoforms described at the UniProt entry for human cardiac troponin T contain the immunogen sequence. Here is the URL for the UniProt page:

    http://www.uniprot.org/uniprot/P45379

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 22 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (RAT cardiac cells)
    Specification
    RAT cardiac cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton x-100 in 3% paraformaldehyde
    Read More

    Tova Volberg

    Verified customer

    Submitted Jul 17 2012

    Question

    The customer claims she submitted the abreview (discount code 40855)

    And did not receive any response

    Please advise

    Read More

    Abcam community

    Verified customer

    Asked on Jul 17 2012

    Answer

    Thank you for your inquiry.

    I am assuming your customer wrote an Abreview for ab45932 and rabbit?

    If so, I can confirm that we received the Abreview (today 17.07.2012)and one of my colleagues is working on it.

    Since the code was not mentioned in the submitted Abreview I added this for your convenience.

    Please thank your customer for their valuable feedback. The code is now active and can be used to purchase further products.

    Read More

    Abcam Scientific Support

    Answered on Jul 17 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (neonatal cardiomyocyte)
    Specification
    neonatal cardiomyocyte
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Mrs. Jamie Soto

    Verified customer

    Submitted Jun 15 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kardiomyocyten)
    Specification
    Kardiomyocyten
    Fixative
    CytoPerm/Fix (BD)
    Permeabilization
    Yes - WashPerm (BD)
    Blocking step
    WashPerm (BD) as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2012

    Question

    Getting nuclear staining as well as expected staining.

    Read More

    Abcam community

    Verified customer

    Asked on May 01 2012

    Answer

    Thank you for calling Abcam earlier today.

    Please let me know if the protocol tips we discussed prove successful or not.

    The antibody is covered under our Abpromise for six months and is guaranteed to work in ICC/IF on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    If there is anything else I can help you with, just let me know.

    Read More

    Abcam Scientific Support

    Answered on May 01 2012

    1-10 of 13 Abreviews or Q&A

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