Anti-Cardiac Troponin T antibody (ab45932)
- Datasheet
- References (20)
- Protocols
Overview
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Product nameAnti-Cardiac Troponin T antibody
See all Cardiac Troponin T primary antibodies -
DescriptionRabbit polyclonal to Cardiac Troponin T
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, Sandwich ELISA, ICC/IF, WBmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Rabbit, Guinea pig, Cow, Cat, Dog
Does not react with:
Mouse -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human cardiac Troponin T.
Read Abcam's proprietary immunogen policy (Peptide available as ab47005.) -
Positive control
- WB: Human fetal heart tissue lysate. IHC-P: Human heart tissue.
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General notes
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab45932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) as Capture. | |
| ICC/IF | 1/400. Incubate for 1-2 hours. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). |
Target
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FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
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Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
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Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
Sequence similaritiesBelongs to the troponin T family.
- Information by UniProt
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Database links
- Entrez Gene: 493940 Cat
- Entrez Gene: 286816 Cow
- Entrez Gene: 403532 Dog
- Entrez Gene: 7139 Human
- Entrez Gene: 100009428 Rabbit
- Entrez Gene: 24837 Rat
- Omim: 191045 Human
- SwissProt: P13789 Cow
see all -
Alternative names
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody (ab45932)This image is courtesy of an Abreview submitted by Dr Silvia Martin-Puigab45932 staining cardiac Troponin T in human fetal cardiomyocytes by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.1% (PBS) saponin buffer and blocked in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 (0.1% saponin, 10% goat serum, 0.01% triton) and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (L+H), diluted 1/600 was used as secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody (ab45932)This image is courtesy of an Abreview submitted by Tova Volbergab45932 staining Cardiac Troponin T in rat cardiac cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in 3% paraformaldehyde. Samples were incubated with primary antibody (1/400 in diluent) for 1 hour at room temperature. A Cy3®-conjugated goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab45932)IHC image of Cardiac Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45932, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-Cardiac Troponin T antibody (ab45932)Anti-Cardiac Troponin T antibody (ab45932) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Sandwich ELISA - Anti-Cardiac Troponin T antibody (ab45932)Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
Protocols
References
This product has been referenced in:
- Nemade H et al. Cell death mechanisms of the anti-cancer drug etoposide on human cardiomyocytes isolated from pluripotent stem cells. Arch Toxicol 92:1507-1524 (2018). Read more (PubMed: 29397400) »
- Bulysheva AA et al. VEGF-B electrotransfer mediated gene therapy induces cardiomyogenesis in a rat model of cardiac ischemia. Bioelectrochemistry 124:105-111 (2018). Read more (PubMed: 30015266) »
