Overview

  • Product name

    Anti-Cardiac Troponin T antibody [EPR20266]
    See all Cardiac Troponin T primary antibodies
  • Description

    Rabbit monoclonal [EPR20266] to Cardiac Troponin T
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Cardiac Troponin T aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P45379

  • Positive control

    • WB: Human fetal heart lysate; Mouse and rat heart lysates. IHC-P: Human, mouse and rat cardiac muscle tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).

Target

  • Function

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities

    Belongs to the troponin T family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on human cardiac muscle is observed [PMID: 9068591].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-Cardiac Troponin T antibody [EPR20266] (ab209813) at 1/5000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Human skeletal muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 36 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Troponin T exists in three tissue-specific subtypes: slow skeletal troponin T1 (ssTnT/TNNT1), fast skeletal troponin T3 (fsTnT/TNNT3) and cardiac troponin T2 (cTnT/TNNT2). The product is specific to cTnT/TNNT2.

    Negative control: human skeletal muscle (PMID: 10585344).

    The observed molecular weight is consistent with the literature (PMID: 10451242).

  • Anti-Cardiac Troponin T antibody [EPR20266] (ab209813) at 1/5000 dilution + Rat heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID: 10451242).

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on human skeletal muscle [PMID: 11375287].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on mouse cardiac muscle is observed [PMID: 9068591].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on mouse skeletal muscle [PMID: 11375287].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on rat cardiac muscle is observed [PMID: 9068591].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on rat skeletal muscle [PMID: 11375287].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Yi Q  et al. Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1. Mol Med Rep 15:2511-2520 (2017). Read more (PubMed: 28447752) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human derived iPSC-Cardiomyocytes)
Permeabilization
Yes - Triton X 0.2%
Specification
human derived iPSC-Cardiomyocytes
Blocking step
FBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Jennifer Furkel

Verified customer

Submitted Feb 18 2019

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