Recombinant Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-6-anti-cardiac-troponin-t-antibody-epr3696-immunohistochemistry-cardiac-muscle-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3696] to Cardiac Troponin T
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cardiac Troponin T antibody [EPR3696]
See all Cardiac Troponin T primary antibodies -
Description
Rabbit monoclonal [EPR3696] to Cardiac Troponin T -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cardiac Troponin T aa 250-350. The exact sequence is proprietary.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3696 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/20000 - 1/100000. Predicted molecular weight: 36 kDa.
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| Notes |
|---|
|
WB
1/20000 - 1/100000. Predicted molecular weight: 36 kDa. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Tissue specificity
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
Involvement in disease
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
Sequence similarities
Belongs to the troponin T family. - Information by UniProt
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Database links
- Entrez Gene: 7139 Human
- Entrez Gene: 21956 Mouse
- Entrez Gene: 24837 Rat
- Omim: 191045 Human
- SwissProt: P45379 Human
- SwissProt: P50752 Mouse
- SwissProt: P50753 Rat
- Unigene: 533613 Human
see all -
Alternative names
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labelling Cardiac Troponin T with purified ab92546 at 1/1000 dilution (0.19 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-468761-anti-cardiac-troponin-t-antibody-epr3696-westernblot-heart-mouse-rat.jpg)
Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)All lanes : Anti-Cardiac Troponin T antibody [EPR3696] (ab92546) at 1/20000 dilution (Purified)
Lane 1 : Rat heart lysate
Lane 2 : Mouse heart lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 15793653).
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![Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-468760-anti-cardiac-troponin-t-antibody-epr3696-westernblot-heart-human.jpg)
Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)Anti-Cardiac Troponin T antibody [EPR3696] (ab92546) at 1/20000 dilution (Purified) + Human heart lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 15793653).
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![Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-3-benefits-of-recombinant-antibodies.png)
Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab92546 has been referenced in 6 publications.
- Park J et al. Modular design of a tissue engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes. Acta Biomater 102:220-230 (2020). PubMed: 31634626
- Cheng Z et al. The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway. Exp Mol Med 50:e447 (2018). WB . PubMed: 29504607
- Chi J et al. Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats. Front Physiol 9:1026 (2018). PubMed: 30108516
- Li H et al. Expression profile of long non-coding RNAs in cardiomyocytes exposed to acute ischemic hypoxia. Mol Med Rep N/A:N/A (2018). PubMed: 30431112
- Chen B et al. Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13:2527-35 (2016). WB ; Mouse . PubMed: 26848028
- Brandt M et al. NOX2 amplifies acetaldehyde-mediated cardiomyocyte mitochondrial dysfunction in alcoholic cardiomyopathy. Sci Rep 6:32554 (2016). IHC-P . PubMed: 27624556