Recombinant Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-Cardiac Troponin T antibody [EPR3696]
See all Cardiac Troponin T primary antibodies -
Description
Rabbit monoclonal [EPR3696] to Cardiac Troponin T -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cardiac Troponin T aa 250-350. The exact sequence is proprietary.
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Positive control
- Human heart lysate; Human skeletal muscle lysate; Mouse heart lysate and Rat heart lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human Heart.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3696 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/20000 - 1/100000. Predicted molecular weight: 36 kDa. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Tissue specificity
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
Involvement in disease
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
Sequence similarities
Belongs to the troponin T family. - Information by UniProt
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Database links
- Entrez Gene: 7139 Human
- Entrez Gene: 21956 Mouse
- Entrez Gene: 24837 Rat
- Omim: 191045 Human
- SwissProt: P45379 Human
- SwissProt: P50752 Mouse
- SwissProt: P50753 Rat
- Unigene: 533613 Human
see all -
Alternative names
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-203530-anti-cardiac-troponin-t-antibody-epr3696-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)IHC image of Cardiac Troponin T staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92546, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-107683-anti-cardiac-troponin-t-antibody-epr3696-western-blot.jpg)
Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)All lanes : Anti-Cardiac Troponin T antibody [EPR3696] (ab92546) at 1/20000 dilution
Lane 1 : Human heart lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Mouse heart lysate
Lane 4 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Datasheets and documents
References
This product has been referenced in:
- Cheng Z et al. The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway. Exp Mol Med 50:e447 (2018). WB . Read more (PubMed: 29504607) »
- Chi J et al. Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats. Front Physiol 9:1026 (2018). Read more (PubMed: 30108516) »
