The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
Use at an assay dependent concentration.
Cardioactive peptide (CCAP) is a cardioregulatory neurohormone that increases heart beat rate during adult wing inflation. This protein has no effect on beat amplitude. The effect of cardioactive peptide is both ino- and conotrophic. Cardioactive peptide has tissue specificity within the central nervous system and most neurons exhibit coexpression with burs.
Immunohistochemistry - Anti-Cardioactive Peptide antibody (ab58736)Image courtesy of an anonymous Abreview.
Drosophila third instar larvae were dissected and fixed to get a fillet preparation. Samples were fixed in formaldehyde, permeabilized using Triton X-100, blocked with 5% serum for 1 hour at 25°C and then incubated with ab58736 at a 1/500 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution. Cardioactive Peptide shows positive staining colocalizing with neuronal marker for HRP.