Overview

  • Product name
    Anti-Cardiotin antibody [R2G]
  • Description
    Mouse monoclonal [R2G] to Cardiotin
  • Host species
    Mouse
  • Specificity
    This antibody reacts with cardiotin, a mitochondrion-associated protein which is present in cardiomyocytes and skeletal muscle.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Goat, Hamster, Cat, Dog, Human, Pig, Xenopus laevis, Monkey, Zebrafish
  • Immunogen

    Total protein extract of chicken gizzard

  • General notes
    Mitochondrial membrane proteins have been shown to be involved in several cellular processes such as the regulation of apoptosis and the energy metabolism. Cardiotin antibodies, which are reactive with mitochondrial proteins, have proven to be early indicators of ischemia induced changes in the myocardium.

Properties

Applications

Our Abpromise guarantee covers the use of ab8962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/25 - 1/500.

Target

  • Relevance
    Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondrial membrane. The cardiotin structure exists as subunits of 60 kDa and 100 kDa. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin, suggesting that the tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myocardium. This monoclonal antibody can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation.

Images

  • Human atrial cardiomyocytes stained with the monoclonal cardiotin antibody R2G.

  • Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.

    Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.
  • Immunofluorescence staining of a 7 days old zebrafish embryo

References

This product has been referenced in:
  • Talavera-Adame D  et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). Read more (PubMed: 19364272) »
  • Dispersyn GD  et al. Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones. Eur Heart J 23:849-57 (2002). Read more (PubMed: 12042006) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you very much for contacting us with your enquiry.

We are able to provide certificates of compliance instead of certificates of analysis, so please find the CoC of the current lot of ab8962 attached to this email.

If there is anything else that we can do for you, please let me know and I'll be happy to help you further.

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Answer

Thank you for your enquiry. I have attached a certificate of compliance for the lot currently in our inventory.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within 120 days of purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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